A directory of core research facilities, services, and instrumentation in Rhode Island

Core Facilities

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3D Facility for Biomedical Sciences: Animation, Visualization and Printing

(last updated 2016-06-16 10:29:30)
»See 3D Facility for Biomedical Sciences: Animation, Visualization and Printing website for more details

Contact: Bongsup Cho, Professor of Biomedical and Pharmaceutical Sciences

Location: Pharmacy Building  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
3D PrinterZ650 3D System/Z-corpProduces realistic color (Powder Based) models without paint to better evaluate the look, feel, and style of product designs. Five print heads provide the widest range of accurate colors, including true black and improved color consistency from part to part. 3D print text labels or images can be directly applied onto printed models.

Benedum Stable Isotope Facility

(last updated 2016-06-16 10:29:30)

Contact: Steven Clemens

Location: Geological Sciences  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Mass Spectrometer, Isotope RatioThermoFisher (Finnigan) MAT 252 dual inlet Isotope Ratio Mass Spectrometer with automated sample reaction device for up to 48 samples and with Kiel III Carbonate interface.Instrument primarily dedicated to the measurement of the stable isotopes of carbon and oxygen on inorganic carbonates. The facility accommodates projects both internal and external to Brown including analysis of terrestrial and marine material. The vast majority of samples analyzed are funded through external grants but I also facilitate a significant number of small unfunded, exploratory, analyses to help construct proof-of-concept data sets in preparation for proposals.

Bioinformatics Core

(last updated 2017-03-08 09:24:04)
»See Bioinformatics Core website for more details

Contact: Christopher Hemme, PhD

Location: Pharmacy Building  »See on the Map

Affiliated Centers: RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Bioinformatics AnalysisService Service to provide bioinformatics consultation, education, and training

Brown University Herbarium

(last updated 2016-06-16 10:29:30)
»See Brown University Herbarium website for more details

Contact: Tim Whitfeld

Location: Biomed Center  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
HerbariumDried and pressed plant specimens mounted on sheets of archival paper. Plant data.The collection includes around 100,000 plant specimens and is an important depository of Rhode Island and New England collections. It is also rich in western and southern North American plants and includes special sets of historically valuable specimens from 19th and early 20th century western US expeditions. Among other important collections, the herbarium also includes a full set of Charles Wright's Cuban plants (1856-1867) and a unique and classic collection of Carex

Bryant Unistructure Science Spaces

(last updated 2016-06-16 10:29:30)

Contact: Dan McNally

Location: Unistructure  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Gas Chromatograph - Mass Spectrometer (GC - MS)
Contact: Julia Crowley-Parmentier
Agilent Technologies 7890A GC and 5975C MSDAnalyze for volatile organics; pyrolysis; MSD and FID
Sample Concentrator, Purge & Trap
Contact: Julia Crowley-Parmentier
Teledyne Tekmar velocity XPT sample concentrator and AQUATek 70 vial autosampleranalyze for highly volatile organics; accessory for the GC-MS
High Performance Liquid Chromatography (HPLC)
Contact: Julia Crowley-Parmentier
ShumatzuAnalyze for non-volatile organics
Spectrometer
Contact: Julia Crowley-Parmentier
Agilent Technologies 7700 series Inductive Coupling Plasma Mass Spectrometer( ICP-MS) with ASX-500 series autosamplerAnalyze for trace elements
Solvent Extractor
Contact: Julia Crowley-Parmentier
Dionex ASE 350 Accelerated Solvent Extractor (ASE)Accelerated extractions
Concentrator Worstation
Contact: Julia Crowley-Parmentier
Caliper LifeSciences Turbo Vap LV Concentration WorkstationConcentrator
Freeze Dry System
Contact: Julia Crowley-Parmentier
Labconco FreeZone 4.5Freeze dryer
Spectrophotometer
Contact: Julia Crowley-Parmentier
Shimadzu UV 2600UV/Vis spectrophotometer for quantifying concentrations
SpectrophotometerThermo Scientific AquaMate PlusUV/Vis spectrophotometer for quantifying concentrations
Microscope - Electron
Contact: Julia Crowley-Parmentier
JEOL JSM 6010LA Scanning Electron Microscope (SEM)Scanning electron microscope with EDS
Critical Point Dryer
Contact: Julia Crowley-Parmentier
Tousimis Sandri-PVT-3D Critical Point Dryer (CPD)Maintain structural integrity for soft tissue use of the SEM
Sputter Coater System
Contact: Julia Crowley-Parmentier
Denton Vacuum Desk V gold sputter coater and carbon coaterCoat non-conducting samples with gold or carbon for use on the SEM
Microscope
Contact: Julia Crowley-Parmentier
Zeiss A1 AXIO Polarized Light Microscope and Q imaging RETIGA 400R ImagerRecordable polarized light imaging
Microscope
Contact: Julia Crowley-Parmentier
Zeiss Axiovert 40CFL Inverted MicroscopeRecordable inverted microscopy
Microscope
Contact: Julia Crowley-Parmentier
Zeiss Primo Star with AxioCamRecordable imaging
Anaerobic Chamber
Contact: Julia Crowley-Parmentier
Microbiology International Whitley DG250 Anaerobic WorkstationSimulate anoxic environments for anaerobic work
Anaerobic Chamber
Contact: Julia Crowley-Parmentier
MBRAUN Labstar Anaerobic Glovebox Workstationsimulate anoxic environments for anaerobic work
PCR Thermal Cycler
Contact: Julia Crowley-Parmentier
Bio-Rad PCR Thermocycler C1000 Touch; Fraction Collector model 2110DNA amplifier
Biological Safety Cabinet
Contact: Julia Crowley-Parmentier
LABGARD ES class II, type A2 Biological Safety CabinetMaintain sterility and contain infectious work
Freezer
Contact: Julia Crowley-Parmentier
Thermo Scientific Isotemp Basic U86-13A41 minus 70 degree freezerLong term storage of biological specimens

CCRI Biology Labs

(last updated 2016-06-16 10:29:30)

Contact: David Vito

Location: Warwick, RI  »See on the Map

Affiliated Centers: RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
AutoclaveConsolidated Stills & Sterilizers 24D36Sterilization. Chamber size is 24inch x36inch, Volume 9.4 cu. Ft
Balance - AnalyticalOrion Analytical Balance Weight determination with capacity of 60g.
Balance - Top LoadingEK-I Compact Balance Weight determination (2 units)
IncubatorFisher Scientific Isotemp Digital & Analog Dry Bath IncubatorProvide controlled dry heat environment for clinical & general chemistry applications
Electrophoresis SystemBio-Rad Mini-Protean Tetra Cell, Vertical protein electrophoresis gel boxesRunning Protein or DNA polyacrylamide gels
MicroscopeLieder MI-500 Series Compound Inverted MicroscopeTissue Culture
pH MeterThermo Scientific Orion 2-Star Bench Top pH MeterGeneral purpose measurements
Blotting System, Semi-Dry Shelton Scientific Semi-Dry Blotting SystemTransfer of protein, RNA & DNA gels to a solid membrane support for visualization, 24cmx30cm.
SpectrophotometerThermo Scientific Genesis 20 Visible SpectrophotometerUV spectrophotometer for quantifying concentrations
TransilluminatorFOTODYNE Foto Phoresis UV TransilluminatorImaging-protein gel analysis, GFP Analysis
Incubator - ShakerLab Companion IS-971 Floor Model Incubator Shaker Growing of bacteria, 250 L chamber
Biological Safety CabinetNuAire Class II Type A2Maintain sterility and contain infectious work

Cell Analysis and Sorting Core

(last updated 2016-06-16 10:29:30)
»See Cell Analysis and Sorting Core website for more details

Contact: Loren Fast, PhD

Location: 80 Washington Street (Shepard Bldg)  »See on the Map

Affiliated Centers: Institute for Immunology and Informatics, COBRE Immune-Based Interventions Against Infectious Diseases

Instrument/ServiceManufacturer/DescriptionFunction
Flow Cytometer
Contact: Alan Rothman
Miltenyi MACSQuant-10 Analytical Flow CytometerAnalytical flow cytometry- 3 lasers, 10 parameters (8 colors, FSC, SSC)
Imaging System - Plate Reader
Contact: Alan Rothman
Biotek Cytation 3Imaging plate reader; 5 colors (brightfield, GFP, RFP, DAPI, Cy5); 10x, 20x, 60x objectives
Microscope
Contact: Alan Rothman
Life Technologies Evos fl, Fluorescence MicroscopeFluorescence microscope; 4 colors (GFP, RFP, DAPI, Cy5); 10x, 40x, 100x objectives
Cell Sorter - Magnetic
Contact: Alan Rothman
Miltenyi AutoMACSAutomated cell sorter for cell suspensions labeled with magnetic beads.
Microplate Reader
Contact: Alan Rothman
Perkin-Elmer EnvisionMultimodal plate reader- absorbance, luminescence, fluorescence, time-resolved fluorescence, AlphaScreen
Flow Cytometer
Contact: Alan Rothman
Analytical flow cytometric analysis; 4 lasers (blue (2), violet (4), red (3) and yellow green (5)) which allows for analysis of up to 14 different fluors (# listed in parentheses for each laser)BD LSRII

Cell Isolation & Organ Function Core

(last updated 2016-06-16 10:29:30)
»See Cell Isolation & Organ Function Core website for more details

Contact: Elizabeth Harrington

Location: The Providence VA Medical Center  »See on the Map

Affiliated Centers: Ocean State Research Institute, CardioPulmonary Vascular Biology COBRE

Instrument/ServiceManufacturer/DescriptionFunction
Analyzer - Cell MetabolismSeahorse Biosciences - XF Extracellular Flux AnalyzerXF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. XF Technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.
Electrical Cell Impedance Sensing (ECIS) equipmentApplied BioPhysics, Model 100Measures cell impedance using altering currents across cell monolayers and measuring the change in electrical potential. Impedance is calculated using Ohm's law.
Electrical Cell Impedance Sensing (ECIS) equipmentApplied BioPhysics, Model ECIS Z ThetaMeasures cell impedance using altering currents across cell monolayers and measuring the change in electrical potential. Impedance is calculated using Ohm's law.
Imaging System - Slide ScannerAperio ScanScope CS Digital Slide Scanner CSBrightfield 5 slide scanner with two workstations with Aperio brightfield imaging analysis software.
Imaging System - Slide ScannerAperio Scan Scope FL Fluorescent Digital Slide Scanner5 slide scanner with Indica Labs fluorescence imaging analysis software.
Flow CytometerMiltenyi MACSQuant Analyzer 7 The MACSQuant Analyzer is a powerful benchtop flow cytometer for highly sensitive, multiparameter cell analysis. MACSQuant Analyzers offer fully automated instrument setup via pre-set calibration and compensation programs. Automated startup, shutdown, and cleaning cycles make for hassle-free instrument housekeeping. The MACSQuantify™ Software provides a powerful and easy-to-use user interface for instrument control, data acquisition, and fully automated analysis.
EvaporatorBuchi RotaVapor R215 EvaporatorRotary evaporator for removing solvents.
Ventilator - MechanicalSCIREQ FlexiventContains a computer-controlled precision piston pump that can intersperse mechanical ventilation with a variety of volume and pressure controlled maneuvers to obtain accurate, reproducible measurement of respiratory mechanics for pulmonary research.
Imaging System - EchocardiographVisualSonics, Vevo 2100 Imaging SystemA high-frequency, high-resolution digital imaging platform with linear array technology and Color Doppler Mode. The Vevo 2100 Imaging System enables in vivo visualization, assessment, and measurement of anatomical structures and hemodynamic function in longitudinal imaging studies for small animal phenotyping.
Channel Cycling Metabolic SystemFor rodent cardiopulmonary exercise Testing OmniTech ElectronicsModel #80 AP4RAT AccuPacer 4 Channel Rat Treadmill and #75 MS8 8

Center for Computation and Visualization

(last updated 2016-06-16 10:29:30)
»See Center for Computation and Visualization website for more details

Contact: Gurcharan Khanna

Location: Center for Computation and Visualization  »See on the Map

Affiliated Centers: Center for Computation and Visualization, RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
High Performance Computing ClusterVarious including IBM idataPlex High Performance Computing ClusterHPC cycles, software development, storage, backups, high speed network hub, visualization, virtual reality, data management, server colocation, scientific support

Centralized Research Core Facility

(last updated 2016-06-29 15:13:03)
»See Centralized Research Core Facility website for more details

Contact: Al Bach

Location: Pharmacy Rm 405  »See on the Map

Affiliated Centers: RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Analyzer - Clinical Chemistry
Contact: Kim Andrews, B.A.
Randox RX Daytona Chemistry AnalyzerClinical chemistry analyzer
Analyzer - Live Cell
Contact: Kim Andrews, B.A.
Roche xCELLigence Real-Time Cell Analysis SystemLabel free live cell analyzer
Biological Safety Cabinet
Contact: Kim Andrews, B.A.
NuAire, Laminar Flow HoodCell culture facility
Cell Counter
Contact: Kim Andrews, B.A.
Nexcellom Cellometer Vision Cell CounterAutomated cell counter
Centrifuge
Contact: Kim Andrews, B.A.
Beckman J2-21M High Speed CentrifugeHigh speed centrifuge
Centrifuge
Contact: Kim Andrews, B.A.
Thermo MultiFuge Centrifuge table-topCentrifuge
Centrifuge - Ultra
Contact: Kim Andrews, B.A.
Beckman Coulter Optima Ultra centrifuge
Centrifuge - Ultra
Contact: Kim Andrews, B.A.
Sorvall Micro Ultra CentrifugeUltra centrifuge
Evaporator
Contact: Kim Andrews, B.A.
Buchi RotaVap SystemRotary evaporator
Fluorescence Activated Cell Sorter (FACS)
Contact: Kim Andrews, B.A.
Becton Dickinson (BD) Biosciences FACS Verse Flow cytometerFACS
Freeze Dry System
Contact: Kim Andrews, B.A.
Labconco 12L Freeze dryerFreeze dryer
Gel Electrophoresis
Contact: Kim Andrews, B.A.
Agilent Offgel Fractionator 3100Liquid phase Isoelectric Focusing (IEF) System
Gel Electrophoresis
Contact: Kim Andrews, B.A.
Bio-Rad 2D Gel SystemTwo Dimensional (2D) Gel Electrophoresis
Gel Electrophoresis
Contact: Kim Andrews, B.A.
Bio-Rad PAGE Electrophoresis SystemOne Dimensional (1D) Gel Electrophoresis
High Performance Liquid Chromatography - Ultra High (UHPLC)
Contact: Kim Andrews, B.A.
Hitachi UPLC SystemUPLC
Chromatography
Contact: Kim Andrews, B.A.
GE AKTA Fast Protein Liquid Chromatograph (FPLC)FPLC
High Performance Liquid Chromatography (HPLC)
Contact: Kim Andrews, B.A.
Hitachi Analytical HPLCHPLC
High Performance Liquid Chromatography (HPLC)
Contact: Kim Andrews, B.A.
Hitachi Prep-HPLC SystemHPLC
Chromatography
Contact: Kim Andrews, B.A.
Teledyne-ISCO CombiFlash SystemFlash Chromatography
Imaging System - Animal
Contact: Kim Andrews, B.A.
Caliper/PE IVIS Lumina Whole Animal ImagerWhole animal imager
Imaging System - Gel Imager
Contact: Kim Andrews, B.A.
GE Imagescanner II Gel imager
Imaging System - Gel Imager
Contact: Kim Andrews, B.A.
GE Typhoon FLA 9000 Multimode ImagerGel imager
Imaging System - Gel Imager
Contact: Kim Andrews, B.A.
Li-Cor Odyssey IR ImagerIR gel imager
Imaging System - Microarray
Contact: Kim Andrews, B.A.
Agilent DNA Microarray ScannerDNA array
Liquid Chromatography - Mass Spectrometer (LC - MS)
Contact: Kim Andrews, B.A.
AB SCIEX TripleTOF 4600 and Shimadzu Prominence UFLC sytemESI-TOF MS
Mass Spectrometer
Contact: Kim Andrews, B.A.
Shimazu Axima MALDI-TOF MS/MSMALDI MS
Mass Spectrometer
Contact: Kim Andrews, B.A.
Thermo iCAPQ-a ICP-MSElemental Analyzer
Microplate Reader
Contact: Kim Andrews, B.A.
BMG LabTech Microplate ReaderFluorescence/UV/VIS
Microplate Reader
Contact: Kim Andrews, B.A.
Molecular Devices SpectraMax M2 Fluorescence/UV/VIS
Microplate Reader
Contact: Kim Andrews, B.A.
Promega GlowMax SystemChemiluminescence
Microscope
Contact: Kim Andrews, B.A.
Nikon Eclipse E600 Upright MicroscopeMicroscope
Microscope
Contact: Kim Andrews, B.A.
Nikon TE2000E /C2 Inverted Confocal Confocal Microscope
PCR Thermal Cycler
Contact: Kim Andrews, B.A.
Applied Biosystems/Life Technologies ABI Vii7- RT-PCRRT-PCR
PCR Thermal Cycler
Contact: Kim Andrews, B.A.
Eppendorf Mastercycler PCRPCR
Protein Sequencer
Contact: Kim Andrews, B.A.
Shimadzu PPSQ-31A protein sequencerN-terminal protein sequencer
Robotic Workstation - Liquid Handler
Contact: Kim Andrews, B.A.
BioTek Precision XS Sample ProcessorSample processor
Robotic Workstation - RNA/DNA
Contact: Kim Andrews, B.A.
Fuji Film DNA/RNA Isolation SystemDNA/RNA isolation
Scintillation Counter
Contact: Kim Andrews, B.A.
PerkinElmer Microbeta1450 Scintillation CounterScintillation counter
Scintillation Counter
Contact: Kim Andrews, B.A.
PerkinElmer Wizard 1470 Gamma CounterGamma counter
Spectrometer
Contact: Kim Andrews, B.A.
PerkinElmer Luminescence Spectrometer LS55Fluorescence
Spectrometer
Contact: Kim Andrews, B.A.
Jasco J-1100 Circular Dichroism (CD) SpectropolarimeteCD
Spectrometer
Contact: Kim Andrews, B.A.
Thermo Nicolet 380 Fourier Transformed Infrared Spectrometer (FT-IR)FT-IR
Spectrophotometer
Contact: Kim Andrews, B.A.
Beckman UV/VIS DU800UV/VIS
Chromatography
Contact: Kim Andrews
Metrohm Ion Chromatography 940 VarioIon Chromatography
Spectrophotometer
Contact: Kim Andrews, B.A.
Thermo Scientific NanoDropUV/VIS
Transfection Platform
Contact: Kim Andrews, B.A.
Lonza Nucleofactor SystemHigh-throughput transfection
Water Purification System - Deionized (DI)
Contact: Kim Andrews, B.A.
Millipore Milli-Q Water SystemDI water System
Western Blotting System
Contact: Kim Andrews, B.A.
Bio-Rad Turbo Trans Blot system Western Blotting
Western Blotting System
Contact: Kim Andrews, B.A.
Life Technologies BenchPro Western SystemWestern Blotting processor
Western Blotting System
Contact: Kim Andrews, B.A.
Protein Simple Simon WesternLiquid phase Western Blotting
Synthesizer, DNA/RNAMerMade-4 Oligonucleotide synthesizerDNA/RNA Synthesizer
High Performance Liquid Chromatography (HPLC)
Contact: Kim Andrews
Shimadzu Nexera-i HPLC systemHPLC
Microscope
Contact: Kim Andrews
EVOS FL Auto Cell Imaging SystemMicroscope
Spectrometer
Contact: Kim Andrews
Jasco Digital Polarimeter P2000Polarimeter

Core Research Laboratories

(last updated 2016-11-22 14:33:01)
»See Core Research Laboratories website for more details

Contact: Suzanne de la Monte

Location: The Coro West Building  »See on the Map

Affiliated Centers: COBRE Center for Cancer Research and Development at Rhode Island Hospital

Instrument/ServiceManufacturer/DescriptionFunction
Cryostat
Contact: Paul Monfils
Reichert-Jung Cryocut 1800Frozen tissue sectioning (used with Superfrost/Plus microscope slides (approximately 4-10 microns).
Fluorescence Activated Cell Sorter (FACS)
Contact: Paul Monfils
Becton Dickinson (BD) Biosciences FACS Vantage Flow CytometerHigh-speed cell sorter. Two lasers analyze cells on 8 parameters. Side/ forward light scatter. 6 different fluorescent color detection. Simultaneously sort up to four populations of cells. Sort 1+ cell type(s) into 6, 24, and 96 well tissue culture plates.
Fluorescence Activated Cell Sorter (FACS)
Contact: Paul Monfils
Becton Dickinson (BD) Biosciences FACSort Flow CytometerAnalytical flow cytometer. Two lasers analyze cells on 6 parameters. Side/ forward light scatter. 4 different fluorescent color detection.
Histology/Tissue Processing - Biosafety Cabinet
Contact: Paul Monfils
Laminar Flow Hood Filter capable of removing 99.97% of particles greater than 0.3 microns.
Histology/Tissue Processing - Knife Sharpener
Contact: Paul Monfils
Hecker Instruments, Inc. Knife SharpenerUsed to sharpen microtome/cryostat knife.
Histology/Tissue Processing - Microwave Oven
Contact: Paul Monfils
Sharp Microwave OvenTissue microwave fixation.
Histology/Tissue Processing - Oven
Contact: Paul Monfils
Fisher Scientific OvenUsed to melt paraffin from paraffin- embedded sections.
Histology/Tissue Processing - Paraffin Embedding Workstation
Contact: Paul Monfils
Miles Scientific Tissue-Tek IIIParaffin embedding sample station (tissue, bone, cell pellet, etc.).
Histology/Tissue Processing - Tissue Processor
Contact: Paul Monfils
Miles Scientific Tissue-Tek-VIP Tissue ProcessorAutomated sample processing (tissue, bone, cell pellet, etc.) for paraffin embedding purposes.
Hybridization Oven
Contact: Carol Ayala
Panomics-Luminex Hybridization ovenHorizontal air flow oven for optimal temperature uniformity.
Imaging System - Camera
Contact: Ginny Hovnesian
Diagnostics SPOT 110-2 Digital Camera Digital Imaging of samples.
Imaging System - Camera
Contact: Ginny Hovnesian
Olympus SPOT IDEA 3.0 MP Digital Camera Digital Imaging of samples. 1.3, 3.0, 5.0 megapixel available. Vivid colors, 12-bit image capture, high-speed readout, external trigger output, USB 2.0 interface for universal connections.
Imaging System - Camera
Contact: Ginny Hovnesian
SPOT RT3 Slider -43C F-MT Digital Camera Attached to Nikon TE-800. Digital Imaging of samples. Ideal for brightfield color imaging, fluorescence microscopy, FISH, GFP imaging, immuno- fluorescence, 3D convolution. CCD cooled to -43.3. 1.92 MP. 1-16x. 14-bit x 20MHz capture, 40MHz live mode, interline progressive scan, FireWire interface.
Imaging System - Dark Room
Contact: Paul Monfils
Dark Room Related equipment eg., automatic processors, dryers, et alSample photo development.
Imaging System - Photo Enlarger
Contact: Carol Ayala
Durst Laborator 1200 Photographic Enlarger Sample digital imaging using high performance enlarger.
Microscope
Contact: Carol Ayala
Bausch Lomb StereomicrosopesVisualization with incident light illumination.
Microscope
Contact: Ginny Hovnesian
NIKON E-800 Fluorescent /Brightfield MicroscopeFluorescence/Brightfield- micro- scope slides only. CFI60 optical system. 0.5x magnification with 50mm field of view. Can be coupled with 2x projection lens to permit macrophotography at 1:1 magnification. Tube lens focal length of 200mm. 10 types of condensers available.
Microscope
Contact: Ginny Hovnesian
Nikon TE 200 Inverted Fluorescent/Brightfield MicroscopeFluorescence/Brightfield Microscope- petri dish/microscope slides on cell/slice culture etc. Phase contrast and fluorescence.
Microscope
Contact: Paul Monfils
Olympus BHTU Upright Light MicroscopeLight Microscope for viewing tissue or cell samples on microscope slides. 45mm parfocal distance system, corrected for a mechanical tube length of 160mm. Two types of standard objectives, D Achromats and D Plan Achromats, magnifications of 4x, 10x, 20x, 40x, 100x. FN20. Equipped for epifluorescence microscopy.
Microscope
Contact: Paul Monfils
Olympus IMT-2 Inverted Light MicroscopeLight Microscope use to view cell/slice/tissue culture samples (microscope slide or petri dish). Inverted research microscope equipped with phase contrast optics.
Microscope
Contact: Carol Ayala
Zeiss S-16 Light MicroscopeLight Microscope used to view 1 micron sectioned resin-embedded samples.
MicroscopeNIKON C1Si Spectral Live Cell Confocal Inverted Microscope System For use with microscope slide. Four diode lasers that excite at 402, 488, 561, & 638 and has spectral capabilities. Suitable for tissue, live cell imaging, time events, FRAP, FRET, deconvolutions, colocalization analysis and 3D reconstructions.
Microscope - Electron
Contact: Carol Ayala
Morgagni 268 Fully Digitized Transmission Electron Microscope (TEM)Ultrastructural analysis (tissue, cells) on stained ultra-thin sections contrasted with uranyl acetate and lead citrate. Examined with Morgagni 268 transmission electron microscope that is fully digitized, high performance, 100 kv, and images collected with an AMT Advantage 542 CCD camera system.
Microtome
Contact: Carol Ayala
Bausch Lomb UltramicrotomeSectioning resin-embedded tissue; micron-nm thickness.
Microtome
Contact: Paul Monfils
Reichert-Jung MicrotomeParaffin embedded tissue sectioning (approximately 2-20 microns).
Vibratome
Contact: Carol Ayala
Bausch Lomb VibratomesSectioning agarose-embedded tissue.
Vibratome
Contact: Paul Monfils
Vibratome 3000 Sectioning SystemFresh tissue sectioning. Thickness: 3- 20 microns.

Electron Microprobe Facility

(last updated 2016-06-16 10:29:30)
»See Electron Microprobe Facility website for more details

Contact: Joseph S Boesenberg

Location: Geo-Chem Building  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Analyzer - Electron Microprobe
Contact: Stephen Parman
Cameca SX100 Electron Microprobe Analyzer The Cameca SX-100 electron microprobe is equipped with 5 wavelength dispersive spectrometers (WDS) and a Rontec energy dispersive spectrometer (EDS).The WDS spectrometers each contain two or four diffracting crystals and presently include LTAP, TAP (2), LPET (2), PET, LLIF (2), LIF, LPCO, PC1 and PC2. The last three crystals are used to measure the light elements (boron to sodium). The absolute detection limits for the instrument are approximately 50-200 ppm, depending on the element. The EDS spectrometer is capable of detecting all elements above sodium (Na). X-ray element maps can easily be acquired on the microprobe. Using 512x512 pixel maps, up to nine element maps (5 elements on WDS, 4 with EDS), plus a backscattered electron (BSE) image, can be obtained simultaneously. Maximum resolution on any one map is one micron per pixel. Utilizing the coordinate system, maps of entire thin sections can be generated.

Environmental Chemistry Facility

(last updated 2016-06-16 10:29:30)
»See Environmental Chemistry Facility website for more details

Contact: David Murray, PhD

Location: MacMillan Hall  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Analyzer - Discrete AnalyzerWestco Smartchem 200Used to determine Nitrate/Nitrite, Phosphate, Ammonia, Silicate, and other nutrient concentrations in water samples and soil extracts.
Analyzer - Elemental AnalyzerCE Elantech, Carbon-Nitrogen NC2100Analysis of carbon, nitrogen, and sulfur concentration of solid samples
Analyzer - Inorganic Carbon AnalyzerUIC, INC Inorganic Carbon AnalyzerMeasures total inorganic carbon by automated acidification and coulometric detection in a wide variety of sample matrices. Solid samples ranging from low ppm to 100% inorganic carbon can be analyzed with this system.
Analyzer - Mercury AnalyzerMilestone DMA-80 Direct Mercury Analyzer Determines mercury content of solid and liquid samples.
Analyzer - Water Isotope Picarro L1102-I Measures delta deuterium/hydrogen (D/H) ratio and delta 18O/16O in liquid water
Freeze Dry SystemLabconco FreeZone 6 Liter For removing water and other solvents from frozen samples by sublimation.
Liquid Chromatography SystemDionex DX500Ion chromatography analysis of ions in marine and freshwater samples
Microwave Digestion SystemMilestone UltrawaveUsed to digest solid samples (soils, sediments, rocks, plants, tissues), for elemental analysis.
Spectrometer - Atomic Absorption Spectrometer-Graphite Furnace (GFAAS)Perkin Elmer AAnalyst 600 Graphite Furnace This spectrometer is useful for measuring trace levels of metals in water and soil.
Spectrometer - Atomic Emission Spectrometer (AES)Jobin Yvon JY2000 Ultrace Inductively coupled plasma atomic emission spectrometer (ICP-AES) Analysis of most elements with atomic weights above 10.
X-Ray Fluorescence - HandheldInnov-X Systems Alpha 4000 Handheld Non-destructive elemental analysis on a variety sample types for concentrations of some metals ranging from about 20 ppm to 10%.
X-Ray Fluorescence - HandheldOlympus Delta Professional HandheldNon-destructive elemental analysis on a variety sample types for concentrations of elements greater than 10 ppm.

Flow Cytometry and Sorting Facility

(last updated 2017-02-20 12:05:03)
»See Flow Cytometry and Sorting Facility website for more details

Contact: Kevin Carlson

Location: Room 602, BioMedical Center Building  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Fluorescence Activated Cell Sorter (FACS)Becton Dickinson (BD) Biosciences FACS Aria IIICell counting, sorting, biomarker detection

Genomics & Sequencing Center

(last updated 2016-06-16 10:29:30)
»See Genomics & Sequencing Center website for more details

Contact: Paul W. Johnson, Manager

Location: 352 Center for Biotechnology & Life Sciences  »See on the Map

Affiliated Centers: RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
Bioanalyzer
Contact: Janet A. Atoyan, Genomics Technician
Agilent Bioanalyzer 2100Provides fast and automated separation, sizing and quantification of DNA, RNA and proteins by miniaturized Lab-on-a-Chip electrophoresis
Bioinformatics Analysis - DNA Sequence and Protein Analysis
Contact: Janet A. Atoyan, Genomics Technician
Software: DNAStar Lasergene Core SuiteComprehensive suite of software modules for DNA, RNA, and protein sequence analysis
Bioinformatics Analysis - Next Generation DNA Sequence Assembly
Contact: Janet A. Atoyan, Genomics Technician
Software: CLCbio Genomics Workbench Software for analyzing, comparing, and visualizing Next Generation Sequencing data
Centrifuge
Contact: Janet A. Atoyan, Genomics Technician
Beckman Coulter Allegra 25R Refrigerated CentrifugeCentrifuge for pelleting cells, tissues and macromolecules
Centrifuge
Contact: Janet A. Atoyan, Genomics Technician
Thermo Savant DNA110 SpeedVac Vacuum CentrifugeCentrifugal concentration system for drying low-volume ethanol or isopropanol precipitates of DNA and RNA
Cryostat
Contact: Janet A. Atoyan, Genomics Technician
Vibratome 5000 Advanced CryostatPerforms histological sectioning of unfixed frozen cells and tissues.
Disrupter
Contact: Janet A. Atoyan, Genomics Technician
Covaris S220 High Performance UltrasonicatorAutomated acoustic disruption and homogenization of cells and tissue samples using focused ultrasonic energy at 500 KHz. Performs DNA Shearing
Disrupter
Contact: Janet A. Atoyan, Genomics Technician
QIAGEN TissueLyserProvides rapid and efficient disruption of cells and tissues
DNA Sequencer
Contact: Janet A. Atoyan, Genomics Technician
Applied Biosystems 3130xl & 3500xl Genetic AnalyzersDNA Sanger Sequencing & Fragment Analysis: Fluorescence-based instruments utilizing capillary electrophoresis for DNA sequencing and fragment analysis
DNA Sequencer
Contact: Janet A. Atoyan, Genomics Technician
Illumina MiSeq DNA NextGen SequencingPerforms clonal amplification, genomic DNA sequencing, and data analysis with base calling, alignment, variant calling, and reporting
Electrophoresis SystemSage Science BluePippin DNA Electrophoresis SystemA preparative electrophoresis platform that separates and extracts DNA fragments using pre-cast gel cassettes
Fluorometer
Contact: Janet A. Atoyan, Genomics Technician
Invitrogen QubitFluorometer that uses fluorescent dyes bound to specific molecules to quantify DNA, RNA and proteins
Imaging System - Camera
Contact: Janet A. Atoyan, Genomics Technician
Zeiss AxioCam HRc high-resolution imaging systemsA digital Zeiss AxioCam HRc high-resolution camera and for laser scanning microscopy with a Zeiss LSM 700 confocal module. For use on the AxioImager upright light microscope
Library Preparation System
Contact: Janet A. Atoyan, Genomics Technician
IntegenX Apollo 324 System NextGen Sequencing (NGS) Library Preparation SystemAutomated liquid handling instrument dedicated to the preparation of sample libraries for Next Generation Sequencing
Microarray Platform
Contact: Janet A. Atoyan, Genomics Technician
BioLog OmniLog Phenotype Microarray System Plate Incubator/ReaderUses Phenotype Microarrays in microtiter plates to test for unique phenotypes or cell functions
Microscope
Contact: Janet A. Atoyan, Genomics Technician
Zeiss AxioImager M2 Upright Light MicroscopeFor use with the AxioImager upright light microscope equipped for digital image acquisition with a Zeiss AxioCam HRc high-resolution camera and for laser scanning microscopy with a Zeiss LSM 700 confocal module
PCR Thermal Cycler
Contact: Janet A. Atoyan, Genomics Technician
Agilent Mx3005P and Roche LC480 qPCR Systems3 Quantitative PCR (qPCR) instruments. Used for highly accurate qualitative and quantitative detection of nucleic acids and gene expression .
PCR Thermal Cycler
Contact: Janet A. Atoyan, Genomics Technician
Eppendorf Mastercycler ep Gradient S and MJ Research PTC-100(3) Thermal cyclers in 96-well format; peltier elements control the temperature of the block for PCR chemistry
PCR Workstation
Contact: Janet A. Atoyan, Genomics Technician
AirClean Systems 600 PCR WorkstationCombination workstation provides an ISO 5 (FS209E) Class 100 clean air environment and UV light sterilization
Robotic Workstation - Automated Pipetting
Contact: Janet A. Atoyan, Genomics Technician
Eppendorf epMotion 5075 VAC Pipetting WorkstationAutomated pipetting workstation enables fast and reproducible liquid handling for a variety of applications
Robotic Workstation - Sample Preparation
Contact: Janet A. Atoyan, Genomics Technician
QIAGEN QIAcube Spin Column RobotAutomated protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins
Spectrophotometer
Contact: Janet A. Atoyan, Genomics Technician
Thermo Scientific NanoDrop 8000Spectrophotometer that accurately measures up to eight individual 1-2 microliter samples simultaneously

Genomics Facility

(last updated 2017-02-20 11:57:26)
»See Genomics Facility website for more details

Contact: Christoph Schorl, PhD

Location: Room 109, Laboratories for Molecular Medicine  »See on the Map

Affiliated Centers: Center for Genomics and Proteomics (CGP), COBRE Center for Signaling Networks at Brown University

Instrument/ServiceManufacturer/DescriptionFunction
Bioanalyzer
Contact: Christoph Schorl
Agilent Bioanalyzer 2100Microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells
Capillary Electrophoresis
Contact: Christoph Schorl
Advanced Analytical, Fragment AnalyzerAnalyze DNA fragments for Next-Gen Sequencing, genotyping, checking RNA quality and quantity, and working with microsatellite simple sequence repeats (SSRs).
Cell Counter
Contact: Christoph Schorl
Life Technologies Automated Countess Cell CounterMeasure cell count and viability (live, dead, and total cells) using the standard trypan blue technique.
Centrifuge
Contact: Christoph Schorl
Sorvall Legend T BenchtopLow to medium speed centrifugation
Centrifuge - Ultra
Contact: Christoph Schorl
Beckman Coulter Optima Max High speed centrifugation with MLA130, TLA110 & TLA100 Rotors
Disrupter
Contact: Christoph Schorl
Covaris S220 High Performance UltrasonicatorDNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization
Fluorescence Activated Cell Sorter (FACS)
Contact: Christoph Schorl
Becton Dickinson (BD) Biosciences FACS Calibur Flow cytometry cell analysis and cell sorting
Hybridization Oven
Contact: Christoph Schorl
Agilent G2534A Microarray hybridization oven
Imaging System
Contact: Christoph Schorl
LiCor Odyssey Clx InfraredWestern blot analysis, EMSA, protein arrays, In-Cell Western Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation, and tissue section analysis.
Imaging System
Contact: Christoph Schorl
Molecular Dynamics Typhoon 9410 Imaging system for gel sandwiches agarose and polyacrylamide gels membranes, microplates and microarrays.
Microarray Platform
Contact: Christoph Schorl
Affymetrix Gene Chip Instrument SystemA fully integrated platform for Affymetrix microarrays
Microarray Scanner
Contact: Christoph Schorl
Molecular Devices GenePix Scanner 4000BTwo color microarray scanner
Microplate Reader
Contact: Christoph Schorl
Molecular Devices Spectra Max M5Detects UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP), Glow Luminescence (Lumi)
PCR Thermal Cycler
Contact: Christoph Schorl
Applied Biosystems 7900 Fast Real Time PCR System96- and 384-well fast real time, quantitative PCR formats
PCR Thermal Cycler
Contact: Christoph Schorl
Applied Biosystems viia-7-real-time-systemMedium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput
PCR Thermal Cycler
Contact: Christoph Schorl
Eppendorf Gradient Mastercycler Gradient cycler enables the temperature across the entire block to be varied within a range of up to 20 C; it can be programmed with every temperature step. For use by genomics facility personnel.
Spectrophotometer
Contact: Christoph Schorl
Thermo Scientific Nanodrop One A microvolume spectrophotometer for measuring DNA, RNA, and protein.
Technical Assistance - Afmetrix Microarray
Contact: Christoph Schorl
ServiceService to provide Affymetrix gene expression array service
Technical Assistance - Genomics and DNA Sequencing Training
Contact: Christoph Schorl
ServiceService to provide Genomics and DNA Sequencing Training
Technical Assistance - Next Generation (NextGen) Sequencing (NGS)
Contact: Christoph Schorl
ServiceService to provide Illumina platform based NextGen sequencing
Microplate Reader
Contact: Christoph Schorl
UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescenceBioTek Cytation 3 Cell Imaging Multi-Mode Reader

Hospital-Imaging Research and Education Service (HI-RES) Facility

(last updated 2016-06-16 10:29:30)
»See Hospital-Imaging Research and Education Service (HI-RES) Facility website for more details

Contact: Daniel Dickstein, MD

Location: Main Building, 3rd Floor  »See on the Map

Affiliated Centers: Brown Institute for Brain Science (BIBS), Norman Prince Neurosciences Institute

Instrument/ServiceManufacturer/DescriptionFunction
Image Analysis and Education
Contact: Daniel Dickstein MD FAAP
The Hospital-Imaging Research and Education Service (HI-RES) Facility has two image analysis and education centers, one at Rhode Island Hospital and the other at Butler Hospital. The Rhode Island Hospital site features advanced computing for image analysis, an image processing server, conference room and three offices. The Butler Hospital site consists of two room that house eight computers equipped for image analysis. HI-RES also utilizes Brown University’s high performance computing center (HPC).Research mission: To foster collaborative imaging research both in/within stakeholder departments and also to foster collaborative links to Brown University campus-based researchers This has the potential to include trainees interested in hands-on research projects as well as hospital-based faculty. Education mission: To bolster the interdisciplinary education of hospital-based trainees in the use of imaging, particularly neuroimaging, to advance the understanding of the biological basis of neurodevelopmental disorders This will include didactic sessions, journal clubs and hands-on sessions with trainees, including medical residents and PhD- trainees.

Hospital-Imaging Research and Education Service (HI-RES) Facility (Butler Hospital)

(last updated 2016-06-16 10:29:30)
»See Hospital-Imaging Research and Education Service (HI-RES) Facility (Butler Hospital) website for more details

Contact: Daniel Dickstein, MD

Location: Butler Hospital  »See on the Map

Affiliated Centers: Brown Institute for Brain Science (BIBS), Norman Prince Neurosciences Institute

Instrument/ServiceManufacturer/DescriptionFunction
Image Analysis and Education
Contact: Daniel Dickstein MD FAAP
The Hospital-Imaging Research and Education Service (HI-RES) Facility has two image analysis and education centers, one at Rhode Island Hospital and the other at Butler Hospital. The Rhode Island Hospital site features advanced computing for image analysis, an image processing server, conference room and three offices. The Butler Hospital site consists of two room that house eight computers equipped for image analysis. HI-RES also utilizes Brown University’s high performance computing center (HPC).Research mission: To foster collaborative imaging research both in/within stakeholder departments and also to foster collaborative links to Brown University campus-based researchers This has the potential to include trainees interested in hands-on research projects as well as hospital-based faculty. Education mission: To bolster the interdisciplinary education of hospital-based trainees in the use of imaging, particularly neuroimaging, to advance the understanding of the biological basis of neurodevelopmental disorders This will include didactic sessions, journal clubs and hands-on sessions with trainees, including medical residents and PhD- trainees

Leduc Bioimaging Facility

(last updated 2017-02-20 12:03:46)
»See Leduc Bioimaging Facility website for more details

Contact: Robbert Creton, PhD

Location: Room 121, Sidney Frank Hall  »See on the Map

Affiliated Centers: Center for Genomics and Proteomics (CGP)

Instrument/ServiceManufacturer/DescriptionFunction
Bioinformatics Analysis - Molecular Devices, MetaMorph Software: Molecular Devices, MetaMorph Software for microscopy automation & image analysis
Critical Point DryerLadd Research IndustriesRemoves liquid from biological specimens for SEM
Glow Discharge UnitEmitech K100XAdds a charge to make TEM samples hydrophilic
MicroscopeOlympus MultiPhoton Laser Scanning Deep optical sectioning of fluorescently labeled samples with SIM photoactivation
MicroscopeZeiss Axiovert 200M FluorescenceImaging of samples labeled with fluorescent indicators
MicroscopeZeiss Axiovert 200M FluorescenceImaging of samples labeled with fluorescent indicators
MicroscopeZeiss LSM 510 Meta ConfocalOptical sectioning of samples labeled with fluorescent indicators
MicroscopeZeiss LSM 710 ConfocalOptical sectioning of samples labeled with fluorescent indicators
MicroscopeZeiss Lumar Stereo FluorescenceImaging of samples labeled with fluorescent indicators
Microscope - ElectronHitachi S-2700 Scanning Electron Microscope (SEM) Transmission electron microscopy with PCI Digital Imaging Accessory
Microscope - ElectronPhilips 410 Transmission Electron Microscope (TEM) Scanning electron microscopy with AMT Camera Accessory
MicrotomeReichert UltramicrotomeUltrathin sectioning for TEM
Microtome Diamond Knife SetDiatome DKSUsed on Reichert microtome to cut ultrathin sections for TEM
Sputter Coater SystemEmitech K550Covers an EM specimen with a thin layer of conducting material
Technical Assistance - BioimagingServiceService to provide Bioimaging Technical Assistance
Technical Assistance - Bioimaging TrainingService Service to provide Bioimaging Training
MicroscopeZeiss LSM 800 Confocal Optical sectioning of samples labeled with fluorescent indicators

Magnetic Resonance Imaging Facility

(last updated 2016-06-16 10:29:30)
»See Magnetic Resonance Imaging Facility website for more details

Contact: Michael Worden, PhD

Location: Sidney Frank Hall  »See on the Map

Affiliated Centers: Brown Institute for Brain Science (BIBS)

Instrument/ServiceManufacturer/DescriptionFunction
Magnetic Resonance Imaging (MRI) ScannerSiemens 3 Tesla Prisma fitBrain structure and function in normal and clinical populations as well as studies of other body systems, non-invasive animal imaging and materials science.

Marine Science Research Facility

(last updated 2016-06-16 10:29:30)
»See Marine Science Research Facility website for more details

Contact: Rodrique Spinette

Location: Aquarium Building  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , Graduate School of Oceanography

Instrument/ServiceManufacturer/DescriptionFunction
Analyzer - Dissolved Gas
Contact: Rodrigue Spinette
Bay Instruments Membrane Inlet Mass Spectrometer- Dissolved Gas AnalyzerMass Spectrometer: Quantifies dissolved gases
Analyzer - Multiparameter Water Analyzer
Contact: Rodrigue Spinette
YSI Professional Plus Hand Held Multiparameter Water Meter Measures temperature, salinity, dissolved oxygen (DO), and pH
Analyzer - Nutrient
Contact: Rodrigue Spinette
Lachat QuickChem 8500 Flow Injection Nutrient Auto AnalyzerMeasures dissolved nutrients in water, Ammonia, Phosphate, Nitrite, Nitrate, and Silicate
Aquatic Culture - Algae and Live Feed Room
Contact: Ed Baker
Various manufacturersK-walls, small rearing tanks for live feed, flowing seawater and freshwater
Aquatic Culture - Phytoplankton Culture Wheels
Contact: Ed Baker
Various manufacturersMotorized and water driven, clear plexiglass, ambient and experimental temperature control, can accommodate culture bottles of differing sizes (1-3L)
Dechlorination System - Freshwater
Contact: Ed Baker
Various manufacturers2 Freshwater dechlorination tanks
Disrupter
Contact: Rodrigue Spinette
Biospec Mini Bead BeaterShakes tubes with beads to disrupt cells, DNA , RNA
Disrupter
Contact: Rodrigue Spinette
Branson Digital Ultasonic Liquid SonifierDisrupts cells
Environmental Chamber
Contact: Ed Baker
EnvironAir Environmental Chamber100 sq ft Temperature control, Two range 4-24 C, Two range -4-24 C (to simulate polar conditions), On generator back up and emergency monitoring systems. (4 Units)
Flowing Seawater System
Contact: Ed Baker
Various manufacturersRaw-Ambient temperature and Filtered- Chilled, Heated, Ambient. UV treated, air supply, emergency monitoring systems
Fluorescence Activated Cell Sorter (FACS)
Contact: Rodrigue Spinette
Becton Dickinson (BD) Biosciences Influx Flow Cytometer and Cell SorterMeasures light scatter and fluorescence of cells. Sorts cells based on measured properties.
Fluorometer
Contact: Rodrigue Spinette
Satlantic Fluorescence Induction Relaxation Fluorometer (FIRE)Measures variable fluorescence in phytoplankton
Fluorometer
Contact: Rodrigue Spinette
Turner Designs 10-AU (Chlorophyll) Field Fluorometer Measures extracted or in vivo chlorophyll fluorescence of samples, Field-portable, can be set up for continuous-flow monitoring or discrete sample analyses
Fluorometer
Contact: Rodrigue Spinette
Life Technologies Qubit FluorometerQuantifies DNA, RNA, and protein
Freezer - Walk-in
Contact: Ed Baker
-20 C Walk-In Freezer185 sq ft Long term storage, On generator back up and emergency monitoring systems
Homogenizer
Contact: Rodrigue Spinette
Polytron Homogenizer PT-10-35-GT Breaks down plant and animal tissue for analysis, and performs homogenization, dispersion, mixing, and prepares emulsions
Cell Counter
Contact: Rodrigue Spinette
Sizing and counting of particles suspended in liquid samplesBeckman Coulter Multisizer 4
Imaging System - Camera
Contact: Rodrigue Spinette
Allied Digital Stingray Firewire Microscope Camera Captures video and still images (C-mount)
Analyzer - Fluid Particle Counter and Imager
Contact: Rodrigue Spinette
Fluid Imaging Technologies FlowCam Imaging Particle Analysis: counts and records images of particles in liquid samples
Incubator
Contact: Rodrigue Spinette
Percival Incubator/Growth ChamberTemperature and light controlled incubator for environmental applications
Microplate Reader
Contact: Rodrigue Spinette
Molecular Devices Spectramax M5 Measures Fluorescence Intensity, Absorbance, Time Resolved Fluorescence, Fluorescence Polarization and Luminescence in cuvettes and multiple sized microplates.
Microscope
Contact: Rodrigue Spinette
Nikon inverted MicroscopeMicroscopy: Works with Stingray Camera
Microscope
Contact: Rodrigue Spinette
Olympus SZX16 Stereo MicroscopeMicroscopy. Works with Stingray Camera
PCR Thermal Cycler
Contact: Rodrigue Spinette
Eppendorf Master Cycler Pro Replicates DNA
PCR Thermal Cycler
Contact: Rodrigue Spinette
Stratagene QPCR Mx3000PAnalysis of gene expression, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Quantitative PCR Filters- FAM/Syber Green, Rox, Hex, CY3, CY5
Photoperiod controller
Contact: Ed Baker
Various manufacturersWattstopper controller(Blount), Various individual timers
Pier Access
Contact: Ed Baker
Access to Graduate School of Oceanography pierAccess to pier for deployment, collection, testing. Water depths 18-15 feet to intertidal zone
Radiometer
Contact: Rodrigue Spinette
Biospherical Instruments Quantum Scalar Laboratory (QSL) Radiometer - PAR Light Meter Measures Photosynthetically Active Radiation (PAR)
Shaker
Contact: Rodrigue Spinette
Thermo Max-Q Orbital Shaker Table Orbital shaker with 18x24 in platform and variety of clamps
Shaker
Contact: Rodrigue Spinette
Thermo Max Q Incubated ShakerHeated incubated shaker
Spectrometer
Contact: Rodrigue Spinette
Ocean Optics Jaz SpectrometerOptical sensing instrumentation that measures light wavelengths. Its unique features and expandable platform makes it uniquely suited for field applications, remote sensing, process flow and quality assurance.
Spectrophotometer
Contact: Rodrigue Spinette
Thermo Scientific NanoDrop 1000Microvolume UV-Vis spectrophotometer that quantifies DNA, RNA, and protein from small 1-2 microliter sample size
Tank - Laminar Flow
Contact: Ed Baker
Laminar Flow Tank1000 gal, Seawater or Freshwater, Multiple speeds with precision control. Experimental chamber visable from 5 sides
Tank - Mesocosm
Contact: Ed Baker
Various manufacturers10, 5 ft deep outdoor mesocosms, with available raw ambient seawater flow
Vacuum Manifold
Contact: Rodrigue Spinette
Vacuum Filter Manifold Allows for filtration of 5 ports simultaneously, 25 and 47 mm filter cups available
Worksation - Marine General Wet Lab
Contact: Ed Baker
Various manufacturers3 Buildings, 7,000 sq ft indoor, and nearly unlimited outdoor space, Multiple insulated tank sizes, various sized aquaria, possible to perform simultaneous replicates
Workstation - Marine Pathology and Transgenics Laboratory and Systems
Contact: Ed Baker
Various manufacturersOzone treated effluent and separate effluent drain
MicroscopeEpifluorescence and phase microscopy with digital camera systemNikon Eclipse Ni epifluorescence microscope
Gas Chromatograph - Mass Spectrometer (GC - MS)
Contact: David Adelman
Identifies and enumerates chemical constituents of samplesAgilent Technologies 7890B/5977A GC/MSD
Gel Imager
Contact: Rodrigue Spinette
Fluorescence gel imaging systemCarestream Molecular Imaging Gel Logic 212 Pro
Cell Counter
Contact: Rodrigue Spinette
Sizing and counting of particles suspended in liquid samplesBeckman Coulter Multisizer 4
Microscope
Contact: Rodrigue Spinette
Epifluorescence and phase microscopy with digital camera systemNikon Eclipse Ni epifluorescence microscope
Gel Imager
Contact: Rodrigue Spinette
Fluorescence gel imaging systemCarestream Molecular Imaging Gel Logic 212 Pro

Mass Spectrometer Analytical Facility

(last updated 2016-06-16 10:29:30)

Contact: Soumen Mallick

Location: GeoChemistry, GC040  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Mass Spectrometer
Contact: Alberto Saal
Thermo Scientific Multi-collector inductively coupled plasma mass spectrometer, ICP-MS, Neptune PlusThis is a multi-collector ICP-MS equipped with nine Faraday cups and eight ion counters, which allows us to monitor multiple masses simultaneously providing precise isotope ratio measurement.
Mass Spectrometer
Contact: Alberto Saal
Thermo Scientific Quadrupole inductively coupled plasma mass spectrometer, ICP-MS, X-series-2This is a single collector ICP-MS that allows us to measure precise elemental composition at sub-ppb level concentration.
Laser Ablation
Contact: Alberto Saal
Photon-machines Analyte G2, 193 nm excimer laser ablationThis 193 nm wave-length excimer laser allows us to perform in-situ analysis on a solid sample with a beam diameter as small as 3 m. To determine the elemental abundances the laser is coupled with the single collector inductively coupled plasma mass spectrometer ICPMS and to determine the isotope ratios the laser is coupled with multi collector ICPMS.
Clean lab, Trace metal free
Contact: Alberto Saal
Pico Trace This Clean Lab is equipped with Millipore water filtration system that provides greater than 18MΩ Milli-Q water. The working stations in this Clean Lab meet ISO class 4 standard (equivalent to FS class 10) allowing us to perform ultra-low blank chemical analysis.

Mass Spectrometry Facility

(last updated 2016-06-16 10:29:30)
»See Mass Spectrometry Facility website for more details

Contact: Tun-Li Shen, PhD

Location: MacMillan Hall  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Gas Chromatograph - Mass Spectrometer (GC - MS)Hewlett-Packard (Agilent) GCD 1800C This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass range 450 Da).
Liquid Chromatography - Mass Spectrometer (LC - MS) Agilent 6530 Mass TOF This system consist of Agilent 1260 infinite HPLC and Agilent 1260 infinite nanoglow LC/chipcube nanoelectrospray MS interface, and 6530 Accurate Mass Q-TOF mass spectrometer . This instrument can detect and identify compounds in amounts smaller than two trillionths of a gram (2 picograms), making the Agilent 6530 a powerful tool for food safety, toxicology and other measurements where minute amounts of compounds must be identified.
Mass SpectrometerJEOL JMS-600H double focusing magnetic sector The JMS-600H is a compact double-focusing, high-resolution, magnetic sector mass spectrometer. This instrument is equipped with electron impact ionization (EI), Chemical Ionization (CI), and fast atom bombardment ionization (FAB) for characterization of synthetic organic, inorganic, and biological molecules. This instrument is also used for routine exact mass measurement of organic molecules.
Mass SpectrometerThermo LXQ linear ion trap with Surveyor HPLC systemThe LXQ has capabilities similar to the conventional 3D ion trap such as LCQ Deca. It is based on linear ion trap technology that has faster cycle time. It is capable of data-dependent LC/MSn analysis.
Mass Spectrometer Thermo LCQ Deca XP Max ion trap with Shimadzu HPLC systemThe LCQ Deca XP Max is equipped with electrospray (ESI) and APCI ionization sources. It is capable of mass analysis of a broad range of molecules that are relatively polar, nonvolatile and thermally labile. This instrument is used for routine molecular weight determination of compounds from organic, inorganic, natural products, small proteins and peptides, and oligonucleotides.
Mass SpectrometerThe Bruker autoflex Speed is a linear time-of-flight (TOF) mass spectrometer equipped with a matrix-assisted laser desorption ionization (MALDI) ion source. This instrument is routinely used for analysis of organic molecules, natural products, and macromolecules, including recombinant proteins, oligonucleotides, polysaccharides and synthetic polymers.Bruker Autoflex Speed Linear (L) Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometer (MALDI-TOF MS).
Gas Chromatograph - Mass Spectrometer (GC - MS)This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass range 1050 Da).Agilent 5977A

Media Prep Kitchen

(last updated 2016-06-16 10:29:30)
»See Media Prep Kitchen website for more details

Contact: Kristi Wharton

Location: BioMedical Center Building  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Media Prep Kitchen for DrosophilaServiceThe Brown Fly Facility is dedicated to providing investigators with high quality media for Drosophila research. The media kitchen is equipped with a food service quality steam kettle fitted with stirring unit, a Steris autoclave and a Lancer Washer fitted with custom racks for glass vials. Media is dispensed using Filamatic and Materflex/ Digistalic pumps. In the centralized kitchen, a standard cornmeal, sucrose, yeast medium is prepared and dispensed into different sized culture vessels.  Media is prepared in a steam-jacketed food kettle and pumped into trays of glass vials (10ml), plastic vials (10ml) or into plastic bottles (30ml) depending on research needs. Media is prepared according to a strict protocol that ensures consistency in diet and the absence of any bacterial, fungal or insect contamination. Researchers using glass vials return them to the facility where they are autoclaved, washed, and filled again.

Molecular Biology and Imaging Core

(last updated 2016-06-16 10:29:30)

Contact: Sunil Shaw PhD

Location: Kilguss Research Institute  »See on the Map

Affiliated Centers: COBRE Center for Perinatal Biology at Women and Infants Hospital

Instrument/ServiceManufacturer/DescriptionFunction
Fluorescence Activated Cell Sorter (FACS)Becton Dickinson (BD) Biosciences FACSCantoAnalytical Flow Cytometer: 2 lasers, 6 colors
Gel ImagerBio-Rad Chemidoc XRS The ChemiDoc XRS+ system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.
Gel Imager Bio-Rad Molecular Imager FX systemImager with 50 micron resolution and a 5 order of magnitude linear dynamic range for fluorescence and radioisotopic imaging. You can also image and accurately quantitate chemiluminescent samples.
Magnetic cell sorterMiltenyi AutoMACSAutomated cell sorter, for separating cell suspensions labeled with magnetic beads
Microplate Reader Molecular Devices Spectramax Gemini EM Fluorescence Microplate ReaderEnables top and bottom reading of microplates over a greater range of fluorescence intensitiesall without the use of filters.
Microplate Reader Perkin Elmer TopCount NXT Microplate Scintillation and Luminescence CounterTopCount NXT analyzes two, six, or twelve samples at a time, and counts samples in 24-, 96-, and 384-well microplates, as well as microcentrifuge tubes. It can count gammas, betas, or luminescent samples.
MicroscopeLeica MZ FLIII Stereo Fluorescent dissection microscope Dissection microscope with Leica color camera DC300F.
MicroscopeNikon 80i Upright Fluorescence MicroscopeUpright microscope with color CCD camera Spot Insight 2
MicroscopeNikon TE2000E Inverted fluorescence, A live cell imaging microscope, with cooled CCD camera Roper CoolSnap HQ
MicroscopeZeiss AxioImager M2 Upright Microscope,Upright microscope with color CCD camera AxioCam MRC.
MicroscopeNikon TE2000E Inverted fluorescence,A Multicolor fluorescence widefield microscope, with stage incubator, specialized in live cell imaging
PCR Thermal CyclerApplied Biosystems ABI 750096-well quantitative PCR
SpectrophotometerBio-Rad smartspec 90-260 V, UV/visible benchtop spectrophotometer for nucleic acid and protein quantitation, wavelength range 200800 nm, built-in thermal printer
SpectrophotometerThermo Scientific NanoDrop 2000 A full spectral microvolume UV-Vis analysis spectrophotometer for measuring DNA, RNA, andprotein.
Suspension Array SystemBio-Rad BioPlex 200 Detection and quantification of multiple analytes (proteins and peptides, or nucleic acids) in a single sample volume.
CryostatFrozen tissue sectionsThermo Shandon Cryotome E
MicrotomeParaffin embedded tissue sectionsThermo Shandon Finesse ME
Histology/Tissue Processing - Paraffin Embedding WorkstationTissue processingThermo Shandon Histocenter 2
Centrifuge - CytoThin layer preparation from cell suspensionsThermo Scientific Cytospin 4 Cytocentrifuge
Gel imagerThe ChemiDoc MP system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.Bio-Rad Chemidoc MP
Cell counterThe Auto T4 is a PC-based instrument for one-step 4x bright field image capture and analysis. This state-of-the-art cell counter performs count, size, and Trypan Blue viability analyses that are 10x faster and more accurate than manual counting. This instrument is ideal for cell lines and purified primary cells, including MCF-7 breast cancer cells, PC3 prostate cancer cells, and 1600 other cell types, due to its advanced software that assesses clumpy cells and omits debrisNexcelom Cellometer

Molecular Biology and Nanomedicine Facility

(last updated 2016-06-16 10:29:30)
»See Molecular Biology and Nanomedicine Facility website for more details

Contact: Qian Chen

Location: The Coro West Building  »See on the Map

Affiliated Centers: COBRE Center for Skeletal Repair, Orthopedics Institute

Instrument/ServiceManufacturer/DescriptionFunction
Microplate Reader
Contact: Cherie Charbonneau
Molecular Devices SpectraMax M2e plate readerFeatures top and bottom read capability for absorbance, fluoresence, luminescence, and time-resolved fluorescence.
Hybridization Oven
Contact: Cherie Charbonneau
Fisher Scientific Isotemp hybridization chamber Temperature range of +7C to 85C. Includes a rotisserie.
Shaker
Contact: Cherie Charbonneau
Lab Companion SI-600 Incubated ShakerBacterial culture shaker with a temperature of ambient to +60C.
Luminometer
Contact: Cherie Charbonneau
Promega Glomax 20/20 luminometerwith printerUltrasensitive luminescence detection in a single microcentrifuge tube (1.5ml) format.
PCR Thermal Cycler
Contact: Cherie Charbonneau
Bioer Life ECO thermal cyclerThis thermal cycler has a 96 well format with a 2C/sec ramp rate.
PCR Thermal Cycler
Contact: Cherie Charbonneau
Applied Biosystems ProFlex thermal cyclerThis thermal cycler has a 3x32 well format that allows for 3 separate programs to be run. The ramp rate is 6C/sec.
PCR Thermal Cycler
Contact: Cherie Charbonneau
Applied Biosystems Veriti thermal cyclerThis thermal cycler has a 96 well format with 3.9C/sec ramp rate.
Imaging system
Contact: Cherie Charbonneau
LI-COR Odyssey Classic Infared Imaging systemThis near-infared fluorescent detector can be used for quantitative western blots, gel shift assays, coomassie stains, tissue section and small animal imaging.
Gel Imager
Contact: Cherie Charbonneau
UVP GelCam 310 Bioimaging digital gel documentation systemHigh resolution 2.0 GelCam 310 CCD with manual zoom lens for fluorscent and colorimetric gel imaging. EtBr filter included.
PCR Thermal Cycler
Contact: Cherie Charbonneau
MJ Opticon 2 real-time PCR system.This 96-well system accepts low-profile tubes or plates. There are two fluorophore channels with emission spectra in the 523-543 nm range and 540-700nm.
Incubator
Contact: Cherie Charbonneau
(4) NuAire IR water-jacketed CO2 incubators (NU-8500)This incubator provides an optimal in-vitro growth environment for both cell and tissue culture while minimizing dessication through a closed loop HEPA filtration system.
Biological Safety Cabinet
Contact: Cherie Charbonneau
(4) NuAire Bio Safety Cabinets (Class II, Type A/B3)These biological safety cabinets reduce exposure to airborne biological or particulate chemical agents in low to moderate risk-hazard research and/or drug preparations.
Centrifuge
Contact: Cherie Charbonneau
Sorvall Legend X1R centrifugeProcess samples for diverse laboratory applications with rotors for 1.5ml eppendorf tubes, 15, and 50ml conical tubes. Refrigerated with a max speed of 152000 rpm.
Autoclave
Contact: Cherie Charbonneau
Amerex HVE-50 top loading autoclave sterilizerThis is a 50L, self-contained, portable, top-loading autoclave with auto-exhaust and warming cycles.
Microscope
Contact: Cherie Charbonneau
Nikon Eclipse TS-100F Inverted MicroscopeThis phase contrast microscope used for tissue culture is also equipped with FITC and TRITC filters to capture fluorescent images in SPOT imaging software.
Microscope
Contact: Cherie Charbonneau
Nikon Eclipse 90i Upright Research MicroscopeThis microscope is equipped with two cameras (color and monochromatic) and is ideal for brightfield and/or epi-fluorescent images of whole tissues etc with 4x, 10x, 20x and 40x dry objectives. The filter cubes available are DAPI, FITC, and TRITC.
Flow Cytometer
Contact: Cherie Charbonneau
Becton Dickinson - AccuriThis system is equipped with a blue and red laser, two light scatter detectors, and fluorescence detectors with optical filters optimized for FITC, PE, PerCP, and APC. The user does not need to adjust detector voltages and there is a wide dynamic range of digitally collected data (7.2 decades).
Bioanalyzer
Contact: Cherie Charbonneau
Invitrogen Luminex 200 with xMap technology This is a versatile, efficient standard in multiplexing with the ability to use magnetic or polystyrene beads on up to 100 targets/sample.
pH Meter
Contact: Cherie Charbonneau
Orion pH meter 920pH range of -2 to 19.99 and oxygen range of -2 to 19.99ppm. Autocalibration buffers of 4.01, 7, and 10.01.
pH meter
Contact: Cherie Charbonneau
Hanna Instruments 2209This pH meter features manual pH calibration and temperature compensation.
Microtome
Contact: Cherie Charbonneau
Thermo Scientific Shandon Finesse 325 manual microtomeWith a lightly balanced flywheel it is ideal for routine paraffin sectioning.
Cutter
Contact: Cherie Charbonneau
Buehler-Isomet CutterThe IsoMet Low Speed Cutter is a precision sectioning saw designed for cutting various types of materials with minimal deformation. Its low kerf loss capability and great versatility in holding all types of sample shapes and configurations, gives today's laboratory a precision sectioning tool capable of cutting viturally any material including brittle or ductile metals, composites, cements, laminates, plastics, electronic devices and biomaterials. The Cutter is equipped with a diamond studded carborundum wheel and Buehler Ecomet 4 grinder/polisher with Automet 2 powerhead.
Imaging system
Contact: Cherie Charbonneau
PerkinElmer FMT 4000 Fluorescence Tomography System.The FMT 4000 fluorescence tomography imaging system provides the greatest utility of the FMT Systems with the ability to quantitate up to four fluorophores simultaneously. The system comes with four excitation laser channels (635, 680, 750, and 790 nm) and is useful for a range of applications including oncology, inflammation, and cardiovascular disease. It is ideal for researchers who are well-versed in 3D fluorescence tomography and require the widest range of in vivo applications and imaging agents.
Imaging system
Contact: Cherie Charbonneau
Faxitorn Pre-clinical imaging systemUltra-high resolution x-ray imaging is an important tool in investigatinga variety of disease states. From metastatic cancer to arthritis andosteoporosis, small animal models are commonly used. Both in vivo andex vivo planar imaging is used to screen, track, and evaluate structuralchanges in these studies. Cabinet x-ray systems allow researchers totake images directly in the vivarium to protect the integrity of the colony. These planar imaging systems are less expensive, subject animals tolower doses, and provide faster results than microCT systems.
Incubator
Contact: Cherie Charbonneau
GQF 1502 Sportsman egg incubatorThis is a complete incubator and hatching system with 3 automatically turned racks. It has an accurate digital thermostat with LCD of humidity and temperature.
Homogenizer
Contact: Cherie Charbonneau
Fisher Scientific, Powergen 125 Tissue HomogenizerProcess volumes from 0.03 to 100mL. Variable speed control to 30,000rpm for efficient cell disruption. Disposable plastic generators eliminate sample cross-contamination. Flat-bottom stainless-steel generators process liquid or soft tissues.
Microscope
Contact: Cherie Charbonneau
Olympus SZ61 Zoom StereomicroscopeGreenough design with two optical paths inclined at an inward angle. Magnification range 0.67X to 4.5X; zoom ratio 6.7:1; FN 22. Working distance of 100mm; observation tube angle 45 degrees; right and left eyepiece tubes interlocked; interpupillary distance adjustment 50-76mm; forward positioned zoom adjustment knobs with zoom magnification graduations. With 0.5x cmount for documentation.
Fume Hood
Contact: Cherie Charbonneau
Thermo Scientific Hamilton SafeAire IIChemical hood and ultra-high vacuum system for organic synthesis
Evaporator
Contact: Cherie Charbonneau
Buchi R-124 RotovaporRotary evaporator comes complete with a 45 degree water condenser and a 1L condensing and evaporating flask.
Circulator
Contact: Cherie Charbonneau
VWR Recirculating Chiller, Model 1160AStable cooling for many common heat removal applications such as rotary evaporators and vacuum systems (-10 to 40C).
High Performance Liquid Chromatography (HPLC)
Contact: Cherie Charbonneau
Agilent Technologies, 1260 Infinity HPLC systemA maximum pressure of 600 bar to utilize smaller particle size columns for higher resolution and faster separations.
Disrupter
Contact: Cherie Charbonneau
Qsonica 700W sonicator with 5.5" cup hornThis sonicator has full amplitude control from 1-100%.
Imaging system
Contact: Cherie Charbonneau
Xtreme back illuminated 4MP with x-ray for small animal imaging High sensitivity luminescence, fluorescence, radioisotopic, and radiographic imaging for up to 5 mice at a time. Isofluarne and temperature controlled chamber for the mice.
PCR Thermal Cycler, Digital
Contact: Cherie Charbonneau
RainDrop Digital PCR by RainDanceThe RainDrop is composed of a Source instrument that can generate 10million droplets/sample and the Sense instrument, which can detect whether the PCR reactions are positive or negative for your fluorophore. Multiplexing is possible for the 8 samples that are run at a time on the microfluidics chip.
PCR Thermal Cycler
Contact: Cherie Charbonneau
BioRad CFX96 Real-Time PCR systemThis real-time PCR instrument allows for up to 5-target multiplexing in sample volumes as small as 10ul. It also has a thermal gradient feature in a 96-well format.
Spectrophotmeter
Contact: Cherie Charbonneau
Thermo Scientific NanoDrop 2000cThis is a full spectrum, UV-Vis spectrophotometer used to quantify and assess the purity of DNA, RNA, protein, and more at sample volumes as low as 0.5ul.
Bioreactor
Contact: Cherie Charbonneau
Flexcell FX5000 Tension SystemThis is a computer-regulated bioreactor that uses vacuum pressure to apply either cyclic or static strain to cells cultured on flexible-bottomed culture plates.
Lyophilizer
Contact: Cherie Charbonneau
Labconco FreeZone Plus 2.5L Cascade Benchtop Freeze Dry SystemThis system is for light sample loads with low eutectic points. It can remove up to 1.8L of water in 24hrs and holds 2.5L of ice before defrosting is required.

Molecular Characterization Facility

(last updated 2016-06-16 10:29:30)
»See Molecular Characterization Facility website for more details

Contact: Al Bach, Ph.D

Location: Pharmacy Rm 315  »See on the Map

Affiliated Centers: RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
Nuclear Magnetic Resonance (NMR) SpectrometerAgilent NMRS 500 with OneNMR probeOptimized for small molecule NMR
Nuclear Magnetic Resonance (NMR) Spectrometer
Contact: Prof. David Rowley
Agilent NMRS 500 with OneNMR ProbeNMR Spectroscopy
Nuclear Magnetic Resonance (NMR) SpectrometerBruker Avance III 300 with BBO probeOptimized for small molecule NMR
Mass SpectrometerABSciex Qtrap 4500 Triple Quadrapole LC/MSHas Shimadzu HPLC system attached

Molecular Pathology Core

(last updated 2016-06-16 10:29:30)
»See Molecular Pathology Core website for more details

Contact: Paula Weston, M.A.

Location: Laboratories for Molecular Medicine, Room 207  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Tissue Processor
Contact: Paula Weston, M.A.
TBS ATP-1 Automated Tissue ProcessorTissue processing for paraffin embedding
Paraffin Embedding Center
Contact: Paula Weston, M.A.
Leica EG1160 Paraffin Embedding CenterParaffin embedding
Automated Microtome
Contact: Paula Weston, M.A.
Microm HM 355S Automated MicrotomeParaffin sectioning
Automated Microtome
Contact: Paula Weston, M.A.
Leica RM2265 Automated MicrotomeParaffin sectioning, plastic semi-thin sectioning
Cryostat
Contact: Paula Weston, M.A.
Leica CM3050S CryostatSectioning of fixed or unfixed frozen samples
Vibratome
Contact: Paula Weston, M.A.
Leica VT 1000S VibratomeSectioning of fixed or unfixed samples
-80C Freezer
Contact: Paula Weston, M.A.
Revco -80C FreezerSample storage
Light microscope with a projector
Contact: Paula Weston, M.A.
Nikon Eclipse 50i light microscope with a projectorCollaborative analyses of histological slides
Laser Capture Microdissection
Contact: Paula Weston, M.A.
Arcturus PixCell IIe Laser Capture MicrodissectionCapture of specific cell types for RNA, DNA or protein analyses
Slide scanner
Contact: Paula Weston, M.A.
Aperio ScanScope CS slide scannerThis microscope uses a 20x objective to produce high-resolution digital scans of histological slides. Located on 5th floor of LMM

Molecular Pathology Core Laboratory

(last updated 2016-11-22 14:30:07)
»See Molecular Pathology Core Laboratory website for more details

Contact: Murray Resnick

Location: Aldrich Building , 6th Floor, Rhode Island Hospital Campus  »See on the Map

Affiliated Centers: COBRE Center for Cancer Research and Development at Rhode Island Hospital

Instrument/ServiceManufacturer/DescriptionFunction
BioanalyzerAgilent Bioanalyzer 2100The Agilent 2100 Bioanalyzer system provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform, providing high quality digital data.
Biological Safety CabinetHEPA filter and NuAire laminar flow hood Filter capable of removing 99.97% of particles greater than 0.3 micronss.
Biospecimen Banking - FreezerThermoForma Freezer - 80 C #8606Sample storage
Biospecimen Banking - FreezerThermoForma Freezer -20 C #3801Sample storage
Biospecimen Banking - FreezerThermoForma Refrigerator #3771Sample storage
Biospecimen Banking - RefrigeratorThermoForma Ultra 932 Lo Freezer -80 C (2) Sample storage
CentrifugeEppendorf 5424 MicrocentrifugeAerosol-tight rotor. Max rcf: 20,238 x g. Automatic lid opening following application sequence. Low access height: 23 cm. Short spin, rpm/rcf setting as required
CentrifugeThermoForma refrigerated MicrocentrifugeWorking temperatures from -5 C to +40 C.
CryostatLeica Cryostat CM1850Frozen Tissue Sectioning. UVC disinfection and AgProtect minimizes contamination risk. Optional section waste and vacuum, disposable blade holder.
Histology/Tissue Processing - BalanceMettler (PB303-S) semimicro balanceMass range of 0.01 mg- 120 g.
Histology/Tissue Processing - HistobathFisher Histobath 134Prevents development of microscopic ice crystals and sample distortion.
Histology/Tissue Processing - Microwave OvenAmana (RFS18MPS) Microwave OvenTissue microwave fixation.
Histology/Tissue Processing - Microwave OvenGE Microwave oven (JE1460BF001)Tissue microwave fixation.
Imaging System - CameraPolaroid camera Instant recording of gels.
Imaging System - Slide ScannerAperio ScanScope Slide Scanner CS2Generates full color, high resolution digital images (cells, tissues and tissue arrays). 5 slide capacity. Image analysis software available. Stored and catalogued in searchable image data base.
IncubatorFisher Scientific Dry Bath IncubatorPrecise temperature control for temperatures up to 130 C. Built in temperature sensing probe for improved temperature accuracy and control.
Laser Capture Microdissection SystemArcturus XT Laser Capture Microdissection System Automated IR and UV laser capture. Microdissection and cutting of cells for RNA, DNA and protein isolation. One modular platform with laser capture and cutting. Optional live-cell modules and camera. Any slide type. Wide range of objectives available, 2x-100x dry and 100x oil immersion.
MicroscopeOlympus Fluorescent Microscope BX41Image Pro Imaging Software. Excitation available 330-385nm, 460-490nm, 510-550nm. Magnifications of 20,40, 100, 200 power. Both reflected light and sample fluorescence may be viewed through and recorded at the microscope.
MicroscopeOlympus BX41 General Use MicroscopeOptical system: UIS2. Built in transmitted Koehler illuminator, 6V/30W bulb. Stage height movement by roller guide, stroke per rotation: 36.8mm. Full stroke range: 25mm. FN20, tube inclination 30 degrees. Stage size: 188 x 134 mm.
MicrotomeFinesse 325 Microtome Used to prepare samples for observation under transmitted light or electron radiation. Microtome material varies depending on the specimen being sliced and the desired thickness of the sections cut.
PCR Thermal CyclerBio-Rad C1000 Touch Thermal Cycler The C1000 Touch thermal cycler offers superior performance and a large color touch screen for easy programming. This fully modular platform is able to accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds without tools. Each PCR module has a fully adjustable heated lid that supports a wide range of vessels and sealers, including low-profile and standard-height PCR plates.
pH MeterAccumet pH meter pH meter.
Robotic Workstation - BarcodingVentana Discovery System Automated staining module using bar code technology. Total automation in IHC staining of frozen/ paraffin embedded tissue, FISH and ISH using DNA, RNA or oligonucleotide probes on tissue or microarrays (gene expression).
SpectrophotometerEppendorf BioPhotometer 50 microliter volumes. Automatic calculation of dilution factors. Measures wave lengths: 230, 260, 280, 340, 405, 490, 550, 595, 650 nm. Spectral bandwidth: 5nm at 230-340nm, 7nm at 405-650nm. Photometric random error +/- 0.002A at 0A, +/- 0.005A at 1A.
Suspension Array SystemBeecher Manual Tissue Microarrayer Generates multiple specimen slides that can contain hundreds of individual tissues. Up to 100 tissue cores per paraffin block. Use for same histochemical and molecular detection techniques as with regular sections.
Vacuum OvenNapco Model 5831 vacuum ovenCapacity: 0.45 cu ft. Temp range: 35 C to 200 C. 30-in Hg vacuum capability. Vacuum bleed valves permit pulling of vacuums as high as 30 inches H. Full view observation window with latched door and built in safety to prevent thermal runaway.
WaterbathPolyScience refrigerated/heated (9106) circulating waterbathThese quality baths are ideal for calibration, electrophoresis, rotary evaporation, viscometry, or other applications requiring cooling or heating. Standard Digital Controller Bath features ±0.05°C temperature stability down to -20°C
PCR Thermal CyclerAvailable for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies.Agilent Mx3005P qPCR System
SpectrophotometerThermo Scientific NanoDrop 2000Wide spectral range (190-840nm) for measuring a variety of samples types. Only requires 0.5 - 2 µL of your sample. Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry. No dilutions required, even for highly concentrated samples. Fast measurement time of less than 5 seconds, 10 -15 seconds from sample pipetting to wiping the pedestal clean.
Imaging System- Image Analysis SoftwareAllows processing and analysis of a 2D and 3D (limited) images (brightfield/IHC, fluorescent, other) for qualitative and quantitative in both spatial and frequency domainsMedia Cybernetics Image-Pro Premier

Mouse Transgenic and Gene Targeting Facility

(last updated 2016-06-16 10:29:30)
»See Mouse Transgenic and Gene Targeting Facility website for more details

Contact: Youngwook Ahn

Location: Laboratories for Molecular Medicine  »See on the Map

Affiliated Centers: COBRE Center for Signaling Networks at Brown University, Center for Genomics and Proteomics (CGP)

Instrument/ServiceManufacturer/DescriptionFunction
Biological Safety CabinetNuAire Safety Cabinets Class 2 Type A/B3Transgenics and gene targeting safety cabinets (2 units)
CentrifugeThermo IEC Centra CL3R Refrigerated CentrifugeTransgenics and gene targeting refrigerated centrifuge
Electroporation SystemBio-Rad Gene Pulsar IITransgenics and gene targeting electroporation system
FreezerCustom BioGenic Systems Liquid Nitrogen (LN2) FreezerTransgenics and gene targeting liquid nitrogen freezer
FreezerThermo Fisher Scientific -80 CTransgenics and gene targeting -80 C freezer
Freezer Frigidaire -20 C FreezerTransgenics and gene targeting freezer
Gene TargetingServiceGene targeting services through transfection and microinjection of murine embryonic stem (ES) cells to produce germline knockout (KO) or knockin (KI) mice
IncubatorNuAire CO2 water jacketed Incubators Transgenics and gene targeting incubators (3 units)
MicroinjectorEppendorf FemtoJet Microinjection SystemTransgenics and gene targeting pronuclear injection system
MicromanipulatorEppendorf Transferman NK 2 MicromanipulatorsTransgenics and gene targeting micromanipulators (2 units)
MicroscopeNikon Eclipse TE200 Inverted MicroscopeTransgenics and gene targeting microinjection microscope
MicroscopeNikon Eclipse TS100 (cell culture scope)Transgenics and gene targeting cell culture scope
MicroscopeNikon SMZ1500 Zoom StereomicroscopesTransgenics and gene targeting dissecting microscopes (2 units)
MicroscopeNikon SMZ800 Zoom StereomicroscopesTransgenics and gene targeting surgical microscope
Technical Assistance - KaryotypingServiceService to determine euploidy of murine embryonic stem (ES) cells prior to microinjection
Technical Assistance -Transgenics and Gene TargetingServiceService to provide custom services related to microinjection, ES cell culture, mouse surgery, or mouse reproduction. The core facility will provide services and/or training related to our capabilities that are not specifically covered by our service list. These services are available for an hourly fee. Examples include: production of MEFs (murine embryonic fibroblasts), polar body biopsy, reproductive surgery, blastomere injection, frozen embryo shipment, training
Transgenics and Gene Targeting - Cryopreserved Mouse Embryo RecoveryServiceService to thaw and implant previously cryopreserved mouse embryos
Transgenics and Gene Targeting - Cryopreserved Mouse Embryo StorageServiceService to store cryopreserved mouse embryos in liquid nitrogen (LN2)
Transgenics and Gene Targeting - Derivation of Mouse Embryonic Stem (ES) cell linesServiceService to produce embryonic stem (ES) cells from an existing mouse line key words: murine embryonic stem cells
Transgenics and Gene Targeting - Embryonic Stem (ES) Cell Injection ServiceService to produce chimeric mice for analysis through microinjection of murine embryonic stem (ES) cells into blastocyst stage embryos.
Transgenics and Gene Targeting - Mouse Colony ExpansionServiceService to use superovulation and surgical implantation to produce large cohorts of age matched mice for experimentation or to quickly expand a mouse colony
Transgenics and Gene Targeting - Mouse Embryo Cryopreservation ServiceService to cryopreserve valuable mouse lines for storage or shipment to other investigators.
Transgenics and Gene Targeting - Mouse In vitro Fertilization (IVF) ServiceService to perform in vitro fertilization of mouse embryos
Transgenics and Gene Targeting - Rederivation of Mouse LinesServiceService to rederive mouse lines obtained from a source other than a Brown University approved vendor. Rederived mouse lines may be housed in any campus mouse facility.
Transgenics and Gene Targeting - TransgenicsServiceService to perform pronuclear microinjection of 0.5 dpc mouse embryos to produce transgenic founders on an FVB/N or C57/BL6 background. Custom backgrounds also available.
Water Purification SystemMilli Q Biocel Transgenics and gene targeting water purification
WaterbathThermo Scientific Precision 260 Circulating Waterbath Transgenics and gene targeting circulating waterbath
Transgenics and Gene Targeting - TransgenicsService to perform mouse IVFService
Transgenics and Gene Targeting - TransgenicsService to perform mouse sperm cryopreservationService

Multiplex Facility

(last updated 2016-06-16 10:29:30)
»See Multiplex Facility website for more details

Contact: Suzanne de la Monte

Location: 55 Claverick Street  »See on the Map

Affiliated Centers: Liver Research Center, Center for International Health Research, Center for International Health Research, Liver Research Center, COBRE Immune-Based Interventions Against Infectious Diseases

Instrument/ServiceManufacturer/DescriptionFunction
Behavioral Phenotyping - Four Lane RotarodEmitter-Columbus Rotamex 5 ControllerUsed to assess motor coordination and balance
Behavioral Phenotyping - Morris Water MazeFabricated in house Opaque white pool with platform for behavioral swim test.
Biospecimen Banking - FreezerHHP Barcoding InstrumentBarcode samples for identification. File Pro software.
CentrifugeBeckman- Coulter Allegra 64R Refrigerated CentrifugeFor use with 50 mL conical tubes.
CryostatLeica CryostatFrozen Tissue Sectioning. UVC disinfection and AgProtect minimizes contamination risk. Optional section waste and vacuum, disposable blade holder.
DisrupterSiegen Tissue LyserDisrupts multiple biological samples through high speed shaking in plastic tubes with steel, tungsten carbide, or glass beads. Up to 48 samples can be processed simultaneously.
Imaging SystemApplied Scientific Instrumentation MS-1000XY stage designed to provide a high resolution, and highly repeatable, means of controlling the X and Y position of a microscope stage
Imaging System - CameraOlympus U-CMAD3 cameraConnected to Olympus Inverted CKX31 Fluorescent Microscope. Digital Imaging of samples. 1.3, 3.0, 5.0 megapixel available. Vivid colors, 12-bit image capture, high-speed readout, external trigger output, USB 2.0 interface for universal connections.
Imaging System - CameraSony 3CCD Exwave HAD Digital CameraConnected to Olympus BX60. Digital Imaging of samples. 1.3, 3.0, 5.0 megapixel available. Vivid colors, 12-bit image capture, high-speed readout, external trigger output, USB 2.0 interface for universal connections.
Imaging System - CameraStereologer 2001/3" type DSP 3-CCD PAL color video camera with improved camera sensitivity (F8 at 2000 lx). Resolution of 800 TVL and a high S/N ratio of 61 dB.
Microplate ReaderMolecular Dynamics M5 SpectracountCross talk (96-well): 3X 10-5, sensitivity: <20 attomol ATP per well, <0.2 firely luciferase per well, spectral range: 380-630 nm.
Microplate ReaderPackard-Perkin Elmer TopcountAnalysis of cell proliferation, receptor binding assays, solid phase RIA/LIA, reporter gene assays, 51Cr release assays, HPLC fraction analysis, scintillation proximity assays, etc.
Microplate ReaderMolecular Devices UV Max Microplate Reader96-wellplate reader. Spectral range: 405-630 nm.
MicroscopeOlympus BX60High-resolution digital pictures using conventional manual filter cubes or more versatile automated filter wheels, and can superimpose multi-color pictures
MicroscopeOlympus Inverted CKX31 Fluorescent MicroscopeInverted microscope with the capability of a fluorescence illuminator for GFP; documentation of images with digital imaging and video monitoring. Use for petri dishes, culture flasks, and multi-well plates.
MicroscopeOlympus Inverted Fluorescent microscopeFN22. 9mm stroke, coaxial coarse and fine focusing knobs. Long working distance universal condenser.
PCR Thermal CyclerRoche Lightcycler 4810Exchangeable 96 or 384 multiwell plate format. Ideal for multiplexing and automated workflows. Provides various methods for gene detection, gene expression analysis, genetic variation analysis and array data validation.
Robotic Workstation - DNA ExtractionQUIAGEN Biorobot EZ1Fully automated purification of genomic DNA from human whole blood and viral nucleic acids from serum or plasma samples.
Robotic Workstation - PipettingEppendorf EpMotion 5070Automated pipetting. Four deck positions can hold tips, reagent reservoirs, single tubes, tube racks, micro-plates, or PCR plates with up to 384 wells. Suited for serial dilutions, reagent distribution, transfer from tubes to plates and normalization.
Robotic Workstation - PipettingTecan Genesis 200 RobotAutomated robot for pipetting tasks. Two arms- the LiHa (liquid handler) and the RoMa (robot manipulator). LiHa- conductivity-sensing 8-channel pipetting arm dispenses volumes of 5-1000 L; RoMa- manipulating arm picks up and moves objects on the workstation.
SpectrophotometerThermo Scientific NanoDrop 2000Measures DNA, RNA, and protein concentrations, and purity (A260/A280 ratio). Conc. range= 2 ng/microliter-15,000 ng/microliter; Min Sample size = 0.5 microliter.
Suspension ArrayLuminex MagpixReads magnetic beadbased immunoassays. Automated reading of 96-well plates (~60 min/plate). Multiplex analysis of up to 50 biomolecules in a single sample.
Suspension ArrayBio-Rad BioPlex 100Automated reading of 96-well plates, up to 9,600 data points in about 35 min. Multiplex analysis of up to 100 biomolecules in a single sample via suspension array.
Suspension Array SystemBio-Rad Vacuum manifoldVacuum purification platform for single 96-well plates or individual luer-ended spin columns. Compatible with Bio-Plex 200 suspension array system.

NMR Facility

(last updated 2016-06-16 10:29:30)
»See NMR Facility website for more details

Contact: Russell Hopson, PhD

Location: MacMillan Hall  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Nuclear Magnetic Resonance (NMR) SpectrometerBruker 300 Avance z-BBI probe, (least sensitivte 1H and indirect detection to all broadband nuclei)
Nuclear Magnetic Resonance (NMR) SpectrometerBruker 600 Avance III HD Ascendz-BBFO probe for all nuclei (most sensitive for all nuclei)
Nuclear Magnetic Resonance (NMR) SpectrometerBruker 400 Avance III Ultra-Shield z-BBFO (2nd most sensitive for 1H and broadband nuclei)
Nuclear Magnetic Resonance (NMR) SpectrometerBruker 400 Avance Ultra-Shield z-BBO (3rd best sensitivity for broadband nuclei)

PC - Albertus Magnus Hall Labs

(last updated 2016-06-16 10:29:30)

Contact: Charles Toth

Location: Albertus Magnus Hall  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Environmental Chamber
Contact: Maia Bailey
Percival Scientific 1 unit
Environmental Chamber
Contact: Maia Bailey
Percival Scientific, AR-22L Growth ChamberFor Arabidopsis
Imaging - Camera
Contact: Pat Ewanchuk
Fastec HiSpec1, Low-Light High-Speed CameraInnovative Low-Light High-Speed Camera. Monochrome with 2GB Memory, C-Mount, includes ImageBLITZ Auto Trigger, Burst Trigger Mode, Multi- Sequence Record Mode (1280 x 1024 at 506fps) 2 Units
Microscope - Electron
Contact: Joe DeGiorgis
Philips EM-300 Transmission Electron Microscope (TEM)Transmission electron microscopy
Laboratory - Prep Lab
Contact: Joe DeGiorgis
Electron Microscopy Prep LabLaboratory space for preparing samples for electron microscopy
Microscope - Electron
Contact: Joe DeGiorgis
Amray-1000 Scanning Electron Microscope (SEM)Scanning Electron Microscope equipped with EDEX for elemental analysis and a digital imaging system, a Zeiss Axiophot equipped for bright field, dark field, DIC and fluorescence microscopy with a digital imaging system
Microtome
Contact: Joe DeGiorgis
Porter-Blum MT-2B UltramicrotomePorter-Blum MT-2B UltraMicrotomes, 2 Units
Aquatic System
Contact: Pat Ewanchuk
Aquatic Habitats Glass Tank System and Life Support Controller Glass tank system and life support controller with low water level protection (18-10 gallon tanks)
Visualization- Camera lucida
Contact: James Waters
Drawing tube, for MZ and M series, the object and the drawing surface are visible at the same time, suitable for drawing for left and right handed persons, work is possible in the daylight, documents can be mirrored inLeica
Respiratory physiology- Li-7000 CO2/H2O Analyzer
Contact: James Waters
The LI-7000 is a high performance, dual cell, differential gas analyzer. It uses a dichroic beam splitter and two separate detectors to measure infrared absorption by CO2 and H2O in the same gas stream. The LI-7000 is ideally suited for applications that demand high speed, high precision measurements in absolute and/or differential modes, including plant and insect gas exchange using chamber-based methods, atmospheric-surface flux eddy covariance and Bowen ratio techniques, vertical profiling, and general atmospheric monitoring.?LI-COR Biosciences

PC - Hickey Hall Labs

(last updated 2016-06-16 10:29:30)

Contact: Charles Toth

Location: Hickey Hall  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
DNA Sequencer
Contact: Elisabeth Arevalo
ABI Prism 3100 Genetic AnalyzerUsed for DNA sequencing and fragment analysis. The ABI PRISM 3100 Genetic Analyzer is an automated capillary electrophoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled DNA fragments in one run.
PCR Thermal Cycler
Contact: Charles Toth
Stratagene Mx3000p Real-time PCR Thermal CyclerThis instrument is available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilents qPCR software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation
Fluorescence Activated Cell Sorter (FACS)
Contact: Charles Toth
Flow Cytometer- Accuri C6The BD Accuri C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable.
Cold Room
Contact: Charles Toth
Walk-In Cold Room
Analyzer - Cell Metabolism
Contact: Marla Tipping
Seahorse Biosciencies, XFe Extracellular Flux Analyzers, XFe96Platform for metabolic assays that simultaneously measures the two major energy producing pathways of the cell - mitochondrial respiration and glycolysis - in a microplate, in real-time.
Laboratory - Tissue Culture
Contact: Charles Toth
Tissue Culture LaboratorySpace and instrumentation for tissue culture preparation
Imaging System - Gel Imager
Contact: Yinsheng Wan
Licor Odyssey IR (Infrared) Imaging SystemInstrumentation for quantitating western blots.
Fluorescence Activated Cell Sorter (FACS)
Contact: Nicanor Austriaco
Cellometer Vision Image CytometryThe Vision CBA System combines the simplicity of image cytometry with the power of flow analysis software to offer user-friendly cell-based assays
Spectrophotometer
Contact: Charles Toth
Thermo Scientific NanoDropUV/VIS

PC - Sowa Hall Labs

(last updated 2016-06-16 10:29:30)

Contact: Charles Toth

Location: Sowa Hal  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Microscope
Contact: Joe DeGiorgis
Zeiss LMS 700 Flexible Confocal Microscope Performs fluorescence measurements, creates optical sections of samples and combines them into 3D image stacks.

Plant Environmental Center

(last updated 2016-06-16 10:29:30)
»See Plant Environmental Center website for more details

Contact: Fred Jackson

Location: Building for Environmental Research and Teaching  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Greenhouse, Plant Environmental Center
Contact: Chris Claussen
Plant environmental center with seven separate, computer controlled rooms where conditions like lighting, temperature, and humidity can be fine tuned; an additional 17 controlled plant growth environments provided by growth chambers (15) and walk-in growth rooms (2)A plant environmental center with a suite of supporting facilities, including a classroom, a "headhouse" for potting and other prep work,and growth chambers for plants.

Proteomics Facility

(last updated 2017-04-13 10:30:04)
»See Proteomics Facility website for more details

Contact: Mandar T Naik, PhD

Location: 3rd Floor, Laboratories for Molecular Medicine  »See on the Map

Affiliated Centers: Center for Genomics and Proteomics (CGP), RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
Bioinformatics Analysis - Mascot ServerSoftware: Matrix Science Mascot Search EngineSoftware for protein identification, characterization and quantitation (v.2.5)
Bioinformatics Analysis - PEAKS Studio Software: Bioinformatics Solutions Inc. PEAKS Studio Software for de novo sequencing and database searching (v.7.0)
Bioinformatics Analysis - ProSight PC
Contact: Mandar T Naik
Software: Thermo Scientific ProSight PC Software for Top-Down proteomics analysis (v.2.0)
Bioinformatics Analysis - ProteoIQSoftware: NuSep ProteoIQ Mass Spec Data AnalysisSoftware for mass spectrometry proteomic analysis (v.2.7)
Bioinformatics Analysis - Proteome Discoverer Software: Thermo Scientific Proteome Discoverer Software for database searching and protein quantitation (v.1.4)
CalorimeterMicrocal Isothermal Titration 200 (ITC)Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
CalorimeterMicrocal VP Differential Scanning (DSC)Measurement of heat changes that occur in biomolecules during controlled increase or decrease in temperature.
FermenterInfors MiniforA bench-top (1-5 L) laboratory fermenter and bioreactor for bacteria and yeast.
Fluorometer - SpectrofluorometerHoriba Jobin Yvon FluoroMax-4Fluorescence detection. A heated cell allows temperature studies.
High Performance Liquid Chromatography (HPLC)Agilent 1200 HPLC SystemHigh performance liquid chromatography system with: quaternary pump, thermostatted autosampler, thermostatted fraction collector, and a multi-wavelength UV/Vis detector. It can be used for analytical detection of biomolecules and/or their purification.
Liquid Chromatography SystemGE Healthcare AktaPrime Plus The KTA Prime Plus is a low pressure chromatography system that performs simple purifications of tagged and untagged proteins.
SpectrophotometerJASCO J-815 Circular Dichroism (CD) SpectrometerProtein folding studies, Protein conformational studies, DNA/RNA interactions, Enzyme kinetics, Purity testing of optically active substances, quantitative analysis of pharmaceuticals, natural products chemistry, biochemistry and macromolecules, rapid scanning (time resolved) experiments. Peltier device for temperature studies.
SpectrometerThermo Scientific NanoDrop 8000 A full spectral microvolume (microliter) UV-Vis analysis spectrophotometer for measuring DNA, RNA, and protein of 8 samples simultaneously.
Surface Plasmon Resonance (SPR) SensorGE Healthcare Biosciencees Biacore X 100Label-free protein interaction analysis provides unique and comprehensive data on the interactions between biomolecules. Performs quantitative kinetic analysis (rate constants), quantitative determination of affinity constants, determination of binding specificity (yes/no), and concentration analysis.
Technical Assistance - Proteomics Project Consultation ServiceService to provide Proteomics Technical Assistance
Technical Assistance - Proteomics TrainingServiceService to provide Proteomics Training
Bioinformatics Analysis - Mascot DistillerSoftware for peptide and protein quantitation (v.2.5.1)Software: Matrix Science Mascot Distiller
Bioinformatics Analysis - ScaffoldSoftware for mass spectrometry proteomic analysis (v.4.3.4)Software: Proteome Software Scaffold Q+S

Proteomics Laboratory

(last updated 2017-03-07 10:40:06)
»See Proteomics Laboratory website for more details

Contact: Nagib Ahsan, PhD

Location: The Coro West Building  »See on the Map

Affiliated Centers: COBRE Center for Cancer Research and Development at Rhode Island Hospital

Instrument/ServiceManufacturer/DescriptionFunction
Bioanalyzer
Contact: Nagib Ahsan, PhD
Agilent Bioanalyzer 2100Microfluidics platform (electrophoresis and flow cytometry) for sizing, quantification, and QC of DNA, RNA, proteins, and cells. Sample size= 1-4 microliters.
Bioinformatics Analysis - MASCOT
Contact: Nagib Ahsan, PhD
Software: Matrix Science MASCOT Search EngineSoftware for MS data analysis through statistical evaluation of matches between observed and projected peptide fragments.
Bioinformatics Analysis - ProteoIQ
Contact: Nagib Ahsan, PhD
Software: NuSep ProteoIQ Mass Spec Data AnalysisSoftware for post-analysis of MASCOT results. Protein and peptide probabilities generated and protein relative quantitation is performed by spectral counting. Normalized and with standard deviations.
Bioinformatics Analysis - Workstation
Contact: Nagib Ahsan, PhD
Hardware: HP HPZ 800 Workstation Software (2 workstations available) used in conjunction with ProteoIQ, DXMS, MASCOT analysis.
Gel Imager
Contact: Nagib Ahsan, PhD
Bio-Rad Molecular Imager VersaDocQuantitative digital imaging system for single or multi-color images. Includes multiplexed samples (1-D or 2-D gels), chemiluminescent and chemifluorescent blots, and microplates.
High Performance Liquid Chromatography (HPLC)
Contact: Nagib Ahsan, PhD
Bio-Rad DuoFlow Biologic HPLCMedium-pressure chromatography system. 100/120 V, F10 workstation, mixer, AVR7-3 valve, UV detector with flow cell and filters. Flow rate= 0.01-10 ml/min.
High Performance Liquid Chromatography (HPLC)
Contact: Nagib Ahsan, PhD
Transgenomic Wave DHPLC SystemDetect mutations/ polymorphisms by Denaturing High Performance Liquid Chromatography (DHPLC). Uses SURVEYOR nuclease cleavage. Identifies single nucleotide polymorphisms (SNPs) or small insertions/ deletions.
High Performance Liquid Chromatography (HPLC)
Contact: Nagib Ahsan, PhD
Dionex UltiMate3000 Nano HPLCSingle gradient and x2 dual configurations. Eluted directly into MS. Min detection= fmole; Min flow rate= 20 nL/min; Max pressure = 800 bar.
Liquid Chromatography- Mass Spectrometer (LC-MS)
Contact: Nagib Ahsan, PhD
Thermo Scientific Q Exactive Plus Mass Spectrometer Benchtop LC-MS/MS. Combination of high-performance quadrupole with high resolution, accurate-mass (HR/AM) Orbitrap. Max resolving power= 140,000; max scan speed= 12Hz, Intra-scan dynamic range > 5000:, Quadrupole mass filter.
Mass Spectrometer
Contact: Nagib Ahsan, PhD
AB SCIEX QSTAR XL Mass SpectrometerIdentification and analysis of protein structure/conformational change by deuterium exchange mass spectrometry (DXMS). LC/MS/MS- Ionization by MALDI or ESI after HPLC (Dionex) and analyzed by quadrupole and TOF.
Mass Spectrometer
Contact: Nagib Ahsan, PhD
Brucker UltrafleXtreme: Imaging Mass SpectrometryMALDI-IMS for tissue imaging, intact proteins analysis, protein spatial distribution, glycoproteomics, or biologics (1-2000 Hz repetition rates) of non-injured vs. injured tissues. MALDI/TOF/TOF analysis for proteomic profiling. Minimum diameter = 10 microns.
Robotic Workstation -Liquid Handler
Contact: Nagib Ahsan, PhD
Perkin Elmer JANUS Automated Workstation Automated Sterile Liquid Handler. 2 arm system, with 4- or 8-tip arm plus a 96/384 MDT arm. Arms include 4-tip or 8-tip dispense arms for transfer from tubes to vials to plates.
Spectrophotometer
Contact: Nagib Ahsan, PhD
Amersham Biosciences UV/Visible Spectrophotometer Ultrospec 1100Wavelength range: 200-900 nm. Spectral bandwidth: 5 nm Wavelength accuracy - 2 nm. Wavelength reproducibility: - 0.5 nm.
Spectrophotometer
Contact: Nagib Ahsan, PhD
Thermo Scientific NanoDrop 2000Measures DNA, RNA, and protein concentrations, and purity (A260/A280 ratio). Conc. range= 2 ng/microliter-15,000 ng/microliter; Min Sample size = 0.5 microliter.
Suspension Array System
Contact: Nagib Ahsan, PhD
Bruker ImagePrep matrix sprayerMatrix deposition device for MALDI Imaging. Average droplet size ? 20 microns, all droplet dia. are < 50 microns. Lateral resolution of 50 microns in tissue.
Technical Assistance - 1D SDS PAGE Coomasie
Contact: Nagib Ahsan, PhD
ServiceService to provide 1D gel (SDS-PAGE) Coomasie Staining.
Technical Assistance - 1D SDS PAGE Silver Stain
Contact: Nagib Ahsan, PhD
ServiceService to provide 1D gel (SDS-PAGE) Silver Stain MS compatible.
Technical Assistance - Gel Imaging
Contact: Nagib Ahsan, PhD
ServiceService to provide Gel imaging by VersaDoc.
Technical Assistance - Mass Spectrometry
Contact: Nagib Ahsan, PhD
ServiceService to provide Imaging Mass Spectrometry Data Analysis.
Technical Assistance - Protein Determination
Contact: Nagib Ahsan, PhD
ServiceService to provide Protein Determination (BCA assay).
Technical Assistance - Protein Extraction
Contact: Nagib Ahsan, PhD
ServiceService to provide ReadyPrep Protein Extraction Kit (Sequential Extraction).
Technical Assistance - Protein Extraction
Contact: Nagib Ahsan, PhD
ServiceService to provide ReadyPrep Protein Extraction Kit (Total Protein).
Technical Assistance - Protein In-Gel Digestion
Contact: Nagib Ahsan, PhD
ServiceService to provide In-gel digestion LC/MS analysis and protein database search.
Technical Assistance - Protein In-Solution Digestion
Contact: Nagib Ahsan, PhD
ServiceService to provide In-solution digestion LC/MS analysis and protein database search.
Technical Assistance - Protein Quantification
Contact: Nagib Ahsan, PhD
ServiceService to provide Data interpretation and protein quantification.
Technical Assistance - Protein Sample Clean-up
Contact: Nagib Ahsan, PhD
ServiceService to provide ReadyPrep 2D Cleanup Kit.
Technical Assistance - Proteomics Project Consultation
Contact: Nagib Ahsan, PhD
ServiceService to provide Project consultation with the Director of Proteomics - Subsequent Consultation
Technical Assistance - Proteomics Project Consultation
Contact: Nagib Ahsan, PhD
ServiceService to provide Project consultation with the Director of Proteomics - Initial Consultation

Research Facility

(last updated 2016-06-16 10:29:30)
»See Research Facility website for more details

Contact: John McGeary, PhD

Location: Research Building 32, Room 146  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
AutoclaveTuttnauer Equipment: consumable sterilization
Behavioral Phenotyping - Eye Tracking SystemTobiiUsed to track eye movement
Bioanalyzer Agilent BioanalyzerDNA size & quantification (quality check for downstream analyses, i.e. next gen sequencing)
Capillary Electrophoresis Applied BiosystemsAnalyze fragment length polymorphisms (96/384 format) (2)
CentrifugeEppendorf Refrigerated Centrifuge Alcohol precipitation
Centrifuge EppendorfFor centrifuging 96/384 plates
DNA SequencerIon Torrent/Life Technologies Personal Genome MachineNext Generation Sequencer
Mass Spectrometer Sequenomhigh throughput SNP genotyping, epigenetics
Microarray ScannerIllumina HiScanWhole-genome genotyping, gene expression analysis, methylation analysis
Microplate ReaderBioTekDNA quantification
Microplate ReaderFluidigm End Point Reader High throughput SNP genotyping
PCR Thermal CyclerApplied Biosystems384 well DNA amplification for downstream analysis (i.e. sequenom processes) (2 units)
PCR Thermal CyclerApplied Biosystems96 well DNA amplification for downstream analysis (i.e. fragment length polymorphisms)(2 units)
PCR Thermal CyclerApplied Biosystems Real Time PCRAnalyze single nucleotide polymorphisms (single locus)
PCR Thermal CyclerApplied Biosystems Real Time PCRAnalyze single nucleotide polymorphisms (single locus)
Robotic Workstation - DNA ExtractionChemagen/Perkin Elmer Magnetic Separation ModuleDNA extraction from blood & saliva
Robotic Workstation - Liquid HandlerSequenom Liquid Handler Transfers PCR chemistries to samples (96/384 format)
Robotic Workstation - Liquid HandlerSequenom NanodispenserDispenses PCR product to Sequenom chip
Robotic Workstation - Sample PreparationIontorrent/Life Technologies Enrichment SystemSimplifies the workflows for the Ion PGM system by providing an automated solution for scalable and reproducible template preparation.
Robotic Workstation - Sample PreparationIontorrent/Life Technologies One TouchSimplifies the workflows for the Ion PGM system by providing an automated solution for scalable and reproducible template preparation.
Robotic Workstation - Sample ProcessingThermo Scientific Cap-it-AllCaps tubes to the optimal torque to maintain sample integrity and provide a hands free method to capping and decapping tubes quickly and easily.
Robotic Workstation -Liquid HandlerBeckman Coulter Lab Automation WorkstationAutomated liquid handler
SpectrophotometerThermo Scientific NanoDropDNA quantification
DNA Sequencer
Contact: Kayla Dwyer
Qiagen Pyromark Q96 IDQuantitative analysis of genetic/epigenetic DNA modifications using Pyrosequencing technology

Rhode Island BioBank

(last updated 2016-06-16 10:29:30)
»See Rhode Island BioBank website for more details

Contact: Garrett Sullivan

Location: BioMedical Center Building  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Bioinformatics Analysis - FreezerworksSoftware: Freezerworks version 6.0.02 Sample management software
Biospecimen BankingService Service to store biospecimens at -80 degrees, 4 degrees and in liquid nitrogen. All equipment is connected to back-up generator power and monitored 24/7 by a Rees alarm system.
Biospecimen Banking - Freezer System Taylor Wharton LABS 40K Liquid Nitrogen Storage SystemLiquid nitrogen storage system connected to back-up generator power and monitored 24/7 by a Rees alarm system
Robotic Workstation - Sample PreparationQiagen QiaCubeDNA, RNA or Protein Purification

Rhode Island Nuclear Science Center

(last updated 2016-06-16 10:29:30)
»See Rhode Island Nuclear Science Center website for more details

Contact: Dr. Cameron Goodwin

Location: URI Bay Campus  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Reactor, Research and Test
Contact: Cameron Goodwin
2 Mega-Watt, light water cooled, pool type reactorNeutron and gamma irradiation and experimental set-up, as well as analysis

RIC - Fogarty Life Sciences Labs

(last updated 2016-06-16 10:29:30)

Contact: Eric Hall

Location: Fogarty Life Science  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Knifemaker
Contact: Breea Govenar
LKB 7800 KnifemakerSlide/Sample Preparation
Microtome
Contact: Breea Govenar
Leica RM2165 Motorized Rotary MicrotomeSlide/Sample Preparation
Microtome
Contact: Breea Govenar
TBS Microtome plus CryostatSlide/Sample Preparation
Microscope
Contact: Breea Govenar
American Optical Series 10 Fluorescence MicrostartMicroscopy course
Microscope
Contact: Breea Govenar
Nikon E600 Epifluorescence with DIC/Phase and digital cameraResearch
Microscope
Contact: Breea Govenar
Olympus CKS41 Inverted MicroscopeTissue culture general use
Microscope
Contact: Breea Govenar
Olympus Compound Light MicroscopeGeneral use
Microscope
Contact: Breea Govenar
Olympus FluoView 1000 Laser Scanning Confocal MicroscopeResearch and Microscopy course
Microscope
Contact: Breea Govenar
Olympus Inverted Epifluorescence Microscope with digital cameraTissue culture/Research
Microscope
Contact: Breea Govenar
Zeiss SteREO discovery V8 Microscope with Digital CameraResearch
Wet Lab / Aquarium room
Contact: Breea Govenar
Aquaria and related equipmentResearch
Autoclave
Contact: Breea Govenar
AutoclaveMicrobiology/Research
Biological Safety Cabinet
Contact: Breea Govenar
Labconco class II Biological Safety CabinetUsed for tissue culture
Biological Safety Cabinet
Contact: Breea Govenar
Sterile laminar flow hoodMicrobiology prep
Centrifuge
Contact: Breea Govenar
Eppendorf 5810R Refrigerated CentrifugeUsed for tissue culture
Centrifuge
Contact: Breea Govenar
Sorvall Discovery M120SE Mini UltracentrifugeResearch/Cell fractionation
Centrifuge
Contact: Breea Govenar
Sorvall Superspeed RC2 CentrifugeGeneral use
Centrifuge
Contact: Breea Govenar
Sorvall Superspeed RC5 Plus CentrifugeResearch
Centrifuge
Contact: Breea Govenar
Thermo IEC Centra CL3R Refrigerated CentrifugeGenetics and Cell & Molec courses
Cold Room
Contact: Breea Govenar
Walk-In Cold RoomLarge cold room for experimentation and storage
Dishwasher
Contact: Breea Govenar
DishwasherMicrobiology/Research
Disrupter
Contact: Breea Govenar
French Pressure Cell PressResearch
Disrupter
Contact: Breea Govenar
Polytron Tissue GrinderSlide/Sample Preparation
Disrupter
Contact: Breea Govenar
SonicatorGeneral use and research
Disrupter
Contact: Breea Govenar
Sonicator with cupResearch and graduate courses
Electroporation System
Contact: Breea Govenar
BioRad Micropulser ElectroporatorResearch
Freeze Dryer
Contact: Breea Govenar
ATMO VAC 5009 freeze dryerSlide/Sample Preparation
Freezer
Contact: Breea Govenar
Thermolyne Liquid Nitrogen cryogenic storage systemCell storage
Freezer
Contact: Breea Govenar
Ultralow FreezerUsed for freezing biological samples, 6 units
Gel Imager
Contact: Breea Govenar
InGenius Syngene Gel Documentation SystemResearch
Ice Machine
Contact: Breea Govenar
Ice MachineGeneral use
Imaging System - Dark Room
Contact: Breea Govenar
Imaging System - Dark RoomGel imaging and analysis
Incubator
Contact: Breea Govenar
Non CO2 IncubatorUsed for tissue culture
Incubator
Contact: Breea Govenar
Thermo CO2 Incubators Used for tissue culture, 2 units
Incubator
Contact: Breea Govenar
Refrigerated IncubatorUsed for tissue culture
Laboratory - Tissue Culture
Contact: Breea Govenar
Tissue Culture RoomA room outfitted with tissue culture instrumentation
Microplate Reader
Contact: Breea Govenar
Lenovo Automated Plate ReaderResearch
Microplate Reader
Contact: Breea Govenar
Thermo Labsystems Multiskan MMCC/340 plate readerResearch
Oven
Contact: Breea Govenar
Drying OvenGeneral use
PCR Thermal Cycler
Contact: Breea Govenar
BioRad IQS Real time PCR thermocyclerResearch
PCR Thermal Cycler
Contact: Breea Govenar
Eppendorf Gradient Mastercycler PCR instrumentation, 2 units
Shakers
Contact: Breea Govenar
Incubator/ShakersMultiple units
Spectrophotometer
Contact: Breea Govenar
BioRad SmartSpec PlusResearch
Spectrophotometer
Contact: Breea Govenar
Eppendorf BiophotometerGectrophotometerseral use
Spectrophotometer
Contact: Breea Govenar
Spectronic 21 D UV/VISResearch
Spectrophotometer
Contact: Breea Govenar
Therm Spectronic Genesys UV/VIS Scanning Research, 6 units
Spectrophotometer
Contact: Breea Govenar
Thermo Scientific NanoDrop ND-1000 UV/VIS, 2 units
Water Purification System
Contact: Breea Govenar
Barnstead Still Water PurifierGeneral use for teaching labs
Water Purification System
Contact: Breea Govenar
Millipore DirectQ (X2)Tissue culture
Water Purification System
Contact: Breea Govenar
Millipore NanoPure Diamond 2nd floor research use
Imaging System - X-ray Film
Contact: Breea Govenar
X-ray film processorBlot imaging

RISD Media Resources

(last updated 2016-06-16 10:29:30)
»See RISD Media Resources website for more details

Contact: Neal Overstrom

Location: RISD Auditorium  »See on the Map

Affiliated Centers: RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
Imaging System - Audio|VisualKodak zi 10 Video Camera (8 units)Portable HD pocket camcorder, batteries, adaptor cables, cases, accessories
Imaging System - Audio|VisualH4N ZOOM Audio Recorder (5 Units)High performance portable digital audio recorder, batteries, adaptor cables, cases, accessories
Imaging System - Audio|VisualZOOM Q3 Video Camera (7 Units)Portable palm camcorder, batteries, adaptor cables, cases, accessories
Imaging System - Audio|VisualEPS-SonyPMWEX1R-01 (2 Units)High performance HD CMOS solid state video camera, batteries, portable hard drive, adaptor cables, cases, studio lighting equipment, accessories

RISD Nature Lab

(last updated 2016-06-16 10:29:30)
»See RISD Nature Lab website for more details

Contact: Neal Overstrom

Location: Waterman Building  »See on the Map

Affiliated Centers: RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
Aquatic SystemMarine Ecological Habitats Closed-System Aquarium560 L closed-system recirculating marine holding tanks with temperature control (2 units)
MicroscopePhenom G2Pro Scanning Electron Microscope (SEM)Desktop Scanning Electron Microscope with ProSuite tiling and remote access software
MicroscopeOlympus SZX16 StereomicroscopeLight microscope with 12.5 MP Jenoptik progressive scan camera, computer workstation, graphics and imaging software
MicroscopeOlympus IX51 Inverted Fluorescent/Brightfield MicroscopeFluorescence/brightfield microscope with 12.5 MP Jenoptik progressive scan camera, computer workstation, graphics and imaging software
MicroscopeOlympus CH30 Upright Light Microscope Upright light microscope with 5 MP Jenoptik digital camera, Canon Vixia HFS20 HD video camera, computer workstation, graphics and imaging software (2 units)
MicroscopeOlympus SZ40 StereomicroscopeLight microscope with 5 MP Jenoptik digital camera, computer workstation, graphics and imaging software
MicroscopeOlympus SZ30 Stereomicroscope Light microscope with LED illuminator (24 units)
GPS UnitTrimble Geo 7x Subfoot GPSSurvey-grade high resolution handheld GPS unit with sub-foot accuracy
GPS UnitGarmin eTrex 30 (10 units)Mapping grade gps unit for student use
Videocamera - High SpeedEdgertronic - monochromeHigh speed videocamera for capturing slow motion footage. Capable of Resolutions up to 1280x1024, frame rates at lower resolution up to nearly 18,000 fps
Videocamera - High SpeedEdgertronic - colorHigh speed videocamera for capturing slow motion footage. Capable of Resolutions up to 1280x1024, frame rates at lower resolution up to nearly 18,000 fps
Videocamera - High SpeedPhantom Miro M120Digital high speed video camera, resolution up to 1920x1080 at 800fps
Scanner, 3DArtec SpiderHigh resolution 3D scanner designed specifically for CAD users
Scanner, 3DStructure SensorScanners (3) for use with iPad Air, capable of 3D scanning objects and entire rooms
Workstation, Computer Geographic Information System (GIS)EPC workstations (3)Computer workstations outfitted with ArcGIS, auto cad and the full adobe creative suite

Rodent Behavioral Phenotyping Facility

(last updated 2016-06-16 10:29:30)
»See Rodent Behavioral Phenotyping Facility website for more details

Contact: Kevin Bath, PhD

Location: BioMedical Center Building  »See on the Map

Affiliated Centers: Brown Institute for Brain Science (BIBS)

Instrument/ServiceManufacturer/DescriptionFunction
Behavioral PhenotypingService A high-throughput behavioral testing facility that integrates high-level computer vision, computer learning, and custom built hardware solutions with behavioral testing to automate tracking, cataloging, and analysis of both home cage behavior and standard rodent testing paradigms to characterize preclinical models of disorders, test novel pharmacological and genetic rescue strategies in rodent models.
Behavioral Phenotyping - 4x Operant ChambersMed AssociatesCan be used to train mice on operant tasks (equipped with water dipper system) for reinforcement learning, as well as grid floor and speaker system for fear conditioning experiments
Behavioral Phenotyping - 2x Open FieldFabricated in house Used to monitor activity in novel settings and to assay anxiety-like behavior
Behavioral Phenotyping - 3-chamber Social Preference Fabricated in house Used to assess a mouse preference for being in close proximity to another mouse.
Behavioral Phenotyping - Barnes Maze Fabricated in house Used to assess spatial learning and memory in rodent models.
Behavioral Phenotyping - Cold Swim StressFabricated in house Used to monitor anxiety-like behavior
Behavioral Phenotyping - Cylinder TestFabricated in house Used to assess motor coordination and balance
Behavioral Phenotyping - Early Life Drug Exposure Fabricated in house Used to assess the impact of pharmacological agents on cognitive, motor, and affective development.
Behavioral Phenotyping - Early Life Stress (Maternal Bedding Restriction)Fabricated in house Used to induce later development of anxiety and depressive-like behavior
Behavioral Phenotyping - Early Life Stress (Variable shock)Fabricated in house Used to assess elevated levels of anxiety and depressive-like behavior
Behavioral Phenotyping - Elevated Zero Maze Fabricated in house Used to monitor rodent anxiety-like behavior
Behavioral Phenotyping - Estrous Cycling (Vaginal Cytology)Fabricated in house Used to assess stage of estrous cycle of rodent models.
Behavioral Phenotyping - Exercise Induced AnorexiaFabricated in house Use of running and food restriction to induce lower levels of body fat and food consumption
Behavioral Phenotyping - Five Lane Forced Treadmill Columbus InstrumentsUsed to induce exercise stress to assess motor fatigue and locomotor abilities.
Behavioral Phenotyping - Five Lane RotarodMed AssociatesUsed to assess motor pattern learning and coordination.
Behavioral Phenotyping - Flurothyl Induced SeizuresFabricated in house Used to assess monitored induction of tonic and clonic seizures
Behavioral Phenotyping - Forced Swim Fabricated in house Used to assess depressive-like behavior
Behavioral Phenotyping - Formalin Test (Pain)Fabricated in house Used to assess chronic pain
Behavioral Phenotyping - Grip Strength MeterColumbus InstrumentsUsed to assess grip strength
Behavioral Phenotyping - Hargreaves Method to Assess Allodinia IITCUsed to assess thermal sensitivity and efficacy of analgesics to decease pain sensitivity
Behavioral Phenotyping - Homecage MonitoringFabricated in house Used for long term monitoring of homecage activity of mice. Can provide longitudinal data regarding (eating, resting, grooming, drinking, walking, climbing, rearing, and micromovements). Will be used as a proxy for other more acute tests.
Behavioral Phenotyping - Homecage Wheel Running Lafayette InstrumentsUsed to assess general levels of locomotor activity and as a proxy to measure changes in circadian rhythms.
Behavioral Phenotyping - Hot Plate Analgesia MeterBasilleUsed to assess thermal sensitivity.
Behavioral Phenotyping - Light /Dark BoxFabricated in house Used to assess anxiety-like behavior
Behavioral Phenotyping - Marble BuryingFabricated in house Used to assess anxiety-like behavior and OCD-like behavior
Behavioral Phenotyping - Maternal DeprivationFabricated in house Used to assess anxiety-like behavior
Behavioral Phenotyping - Maternal SeparationFabricated in house Used to assess anxiety-like behavior
Behavioral Phenotyping - Morris Water Maze Fabricated in house Used to assess spatial learning and memory
Behavioral Phenotyping - Non-invasive Blood Pressure MonitoringColumbus InstrumentsTail cuff and apparatus for repeated measurement of blood pressure in mice.
Behavioral Phenotyping - Novel Object IdentityFabricated in house Used to assess working memory
Behavioral Phenotyping - Novel Object LocationFabricated in house Used to assess spatial learning and memory
Behavioral Phenotyping - Novelty Induced HypophagiaFabricated in house Used to assess anxiety-like behavior and translational efficacy of antidepressants and anxiolytic drugs.
Behavioral Phenotyping - Novelty Suppressed FeedingFabricated in house Used to assess anxiety-like behavior and translational efficacy of antidepressants and anxiolytic drugs.
Behavioral Phenotyping - Olfactory Cross-habituationFabricated in house Used to assess olfactory acuity.
Behavioral Phenotyping - Olfactory Perceptual LearningFabricated in house Used to assess ability to learn to discriminate closely related odor pairs.
Behavioral Phenotyping - Predator Odor StressFabricated in house Used to assess anxiety-like behavior
Behavioral Phenotyping - Resident Intruder AggressionFabricated in house Used to assess propensity for aggressive behavior.
Behavioral Phenotyping - Restraint StressFabricated in house Used to assess hippocampal atrophy and increased anxiety and depressive-like behavior.
Behavioral Phenotyping - Righting ReflexFabricated in house Used to assess coordination and ability to right from a prone position.
Behavioral Phenotyping - Shock ThresholdFabricated in house Used to assess changes in pain sensitivity
Behavioral Phenotyping - Single Lane RotarodMed AssociatesUsed to assess motor pattern learning and coordination.
Behavioral Phenotyping - Social Defeat StressFabricated in house Used to assess anxiety-like behavior
Behavioral Phenotyping - Social Disruption StressFabricated in house Used to assess anxiety-like behavior
Behavioral Phenotyping - Social Odor DiscriminationFabricated in house Used to assess ability to detect and discriminate between odorants of conspecifics.
Behavioral Phenotyping - Sucrose PreferenceFabricated in house Used to asses depressive-like behavior (anhedonia).
Behavioral Phenotyping - Tail Suspension Fabricated in house Method to assess depressive-like behavior
Behavioral Phenotyping - Visual Cliff Fabricated in house Used to assess depth perception and avoidance learning during visual development.
Behavioral Phenotyping - von Frey FilamentsBiosebUsed to assess tactile sensitivity
Behavioral Phenotyping - Wire-hangFabricated in house Used to assess animals strength and endurance.

RWU Lab Spaces

(last updated 2016-06-16 10:29:30)

Contact: Nancy Breen

Location: Marine and Natural Sciences  »See on the Map

Affiliated Centers: RI-NSF EPSCoR , RI-INBRE

Instrument/ServiceManufacturer/DescriptionFunction
Flow Cytometer
Contact: Marcie Marston
Millipore Guava easyCyte Flow CytometerFlow Cytometer
Gas Chromatograph - Mass Spectrometer (GC - MS)
Contact: Nancy Breen
Agilent 5890 Gas Chromatograph - Mass Spectrometer (GC - MS) wth Mass Selective Detetor (MSD)GC MS for teaching and research
High Performance Liquid Chromatography (HPLC)
Contact: Nancy Breen
Waters 2695 High Performance Liquid Chromatograph-MS / ZQ Micromass Mass SpectrometerLC - MS for teaching and research
Gas Chromatograph
Contact: Nancy Breen
Agilent 7890 Gas Chromatography with DB-23 columnGC for fatty acid analysis

Salve Regina Biology and Biomedical Sciences

(last updated 2016-06-16 10:29:30)
»See Salve Regina Biology and Biomedical Sciences website for more details

Contact: John-David Swanson

Location: O'Hare Academic Center  »See on the Map

Affiliated Centers: RI-NSF EPSCoR

Instrument/ServiceManufacturer/DescriptionFunction
Flow CytometerMillipore Guava EasyCyte 8 Flow CytometerSingle blue (488 nm), dual blue and red (642 nm), or triple blue, red, and violet (405 nm) excitation lasers provide up to 12 simultaneous detection parameters, including 10 fluorescent colors plus forward and side scatter for size and granularity determination. The guava easyCyte family also meets your sample throughput needs by offering both single sample and multi-sample processing.

Statistics and Data Management Core

(last updated 2016-06-16 10:29:30)
»See Statistics and Data Management Core website for more details

Contact: Jennifer Friedman, MD, PhD

Location: 55 Claverick Street  »See on the Map

Affiliated Centers: Center for International Health Research, COBRE Immune-Based Interventions Against Infectious Diseases

Instrument/ServiceManufacturer/DescriptionFunction
Technical Assistance - Statistics and Data Management
Contact: Sangshin Park, PhD
ServiceThis Core provides consultation and assistance in managing and analyzing research data, such as the large datasets, and in linking these data with clinical predictors and outcomes collected in the field. The Core also performs more sophisticated data analytic approaches, including hierarchical, repeated measures modeling and Principal Components Analysis.

Structural Biology Facility

(last updated 2017-01-26 13:38:54)
»See Structural Biology Facility website for more details

Contact: Mandar T Naik, PhD

Location: Laboratories for Molecular Medicine  »See on the Map

Affiliated Centers: Center for Genomics and Proteomics (CGP)

Instrument/ServiceManufacturer/DescriptionFunction
Nuclear Magnetic Resonance (NMR) SpectrometerBruker 500 Avance II, with TCI cryoprobeAn NMR spectrometer optimized for studying proteins in solution. This spectrometer is most useful for tracking binding interactions, measuring dynamic properties of proteins, and assigning protein resonances. Capable of using 5 mm and 3 mm tubes. Also suitable for proton NMR of small molecules and natural products.
Nuclear Magnetic Resonance (NMR) SpectrometerBruker 850 MHz Avance III HD, with TCI cryoprobeAn NMR spectrometer optimized for studying proteins in solution. This spectrometer is ideal for determining protein structures and measuring protein dynamics. Its extreme sensitivity allows studies on otherwise intractable samples. Capable of using 5 mm, 3 mm, and shaped NMR tubes. Also suitable for proton NMR of small molecules and natural products
X-ray Diffraction SystemRigaku ACTOR robot and Saturn 944 HG CCDAn instrument useful for obtaining X-ray diffraction patterns of proteins and other biomacromolecules under cryogenic or room-temperature conditions. Rapid screening and sorting of up to 80 candidate crystals is possible.
X-ray Scattering SystemRigaku BioSAXS 1000 with Pilatus 100K detectorAn instrument useful for collecting small- and wide- angle X-ray scattering data for proteins in aqueous solution. This instrument is useful for assessing molecular size and shape. Temperature controller available.

Tissue Banking Core

(last updated 2016-11-22 14:32:07)
»See Tissue Banking Core website for more details

Contact: Murray Resnick

Location: Aldrich Building , 6th Floor, Rhode Island Hospital Campus  »See on the Map

Affiliated Centers: COBRE Center for Cancer Research and Development at Rhode Island Hospital

Instrument/ServiceManufacturer/DescriptionFunction
Bioinformatics Analysis - DatabaseSoftware: Database developed in Filemaker ProDatabase and tissue inventory management on a Dell computer workstation
Biospecimen Banking - Biosafety CabinetDissecting hoodHood dedicated to sterile tissue cutting with biohazard protection.
Biospecimen Banking - Freezer System Forma 195878 LN2 backup for bank freezersSystems inject liquid LN2 into cabinet when cabinet temperature warms to preset level, adjustable in 1 C increments to -150 C.
Biospecimen Banking - Freezing BathLow temp isopentane bathBath with min. temperature of -160 C by addition of liquid nitrogen to isopentane.
Histology/Tissue Processing - Freezing BathLow temp isopentane bathBath with min. temperature of -160 C by addition of liquid nitrogen to isopentane.

X-ray Reconstruction of Moving Morphology Facility (XROMM)

(last updated 2016-06-16 10:29:30)
»See X-ray Reconstruction of Moving Morphology Facility (XROMM) website for more details

Contact: Beth Brainerd, PhD

Location: BioMedical Center Building  »See on the Map
Instrument/ServiceManufacturer/DescriptionFunction
Computed Tomography (CT) Scanner Animage Fidex VeterinaryVeterinary CT Scanner designed for animals in the size range from rats to dogs. In vivo scanning of anesthetized animals is possible.
Videoradiography RoomCustom made biplanar3D x-ray motion analysis methods that combine skeletal movement data from in vivo x-ray videos with skeletal morphology from 3D bone scans (e.g. computed tomography (CT) or laser scans).
X-ray FluoroscopesRadiological Imaging Mobile C-Arm X-ray Two retrofitted with 30-cm image intensifiers and high-speed video cameras for measuring accurate 3D skeletal kinematics of small to medium-sized animals.